Identification of chemical constituents in Huangdi Anxiao Capsules by UPLC-Q-TOF-MS~E combined with UNIFI software.
10.19540/j.cnki.cjcmm.20191217.301
- Author:
Jia-Rong GAO
1
;
Meng-Qing ZHU
2
;
Xiao-Li WANG
2
;
Huan WU
3
;
Li SHAN
4
;
Yun WANG
2
Author Information
1. State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine Hefei 230031, China Anhui Province Key Laboratory of Chinese Medicinal Formula Hefei 230012, China.
2. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
3. the Experimental Research Center, Anhui University of Chinese Medicine Hefei 230038, China.
4. State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine Hefei 230031, China.
- Publication Type:Journal Article
- Keywords:
Huangdi Anxiao Capsules;
UNIFI;
UPLC-Q-TOF-MS~E;
chemical constituent
- MeSH:
Capsules;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Mass Spectrometry;
Rhizome;
Software
- From:
China Journal of Chinese Materia Medica
2020;45(10):2395-2405
- CountryChina
- Language:Chinese
-
Abstract:
In order to clarify the main chemical constituents of Huangdi Anxiao Capsules, an ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS~E) combined with Waters UNIFI software were successfully used to rapidly identify the chemical constituents in Huangdi Anxiao Capsules. The mass spectrometry data of chemical constituents from Huangdi Anxiao Capsules were collected by UPLC-Q-TOF-MS~E, and their structures were identified by the results of UNIFI software according to relative retention time of reference standards, MS feature fragments and literature data of each compound. A total of 100 compounds in Huangdi Anxiao Capsules were identified, including 25 compounds from Pueraria Lobate Radix, 22 compounds from Coptis Rhizoma, 6 compounds from Ophiopogonis Radix, 14 compounds from Eriobotryae Folium, 22 compounds from Rehmanniae Radix, and 15 compounds from Notoginseng Radix et Rhizoma. Among them, 3 compounds were common components. These 100 compounds included flavonoids, alkaloids, saponins and organic acids. This study systematically analyzed the chemical composition of Huangdi Anxiao Capsules, so as to provide evidences for defining the chemical material basis of Huangdi Anxiao Capsules.
