Early intervention of bone-nearby acupuncture combined with electroacupuncture on morphine tolerance in bone cancer pain rats and its effect on the expression of HDAC and MOR in dorsal root ganglia.
10.13703/j.0255-2930.20190822-k0002
- Author:
Xue-Mei ZHONG
1
;
Jun-Fan FANG
2
;
Bin JIANG
3
;
Jie ZHOU
2
;
Yang-Qian CAI
2
;
Yi LIANG
2
;
Jian-Qiao FANG
2
;
Feng CHEN
1
;
Jun-Ying DU
2
Author Information
1. Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Acupuncture and Moxibustion, First Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang Province.
2. Third Clinical Medical College, Zhejiang Chinese Medical University, Zhejiang Key Laboratory of Acupuncture and Moxibustion Neurology Research, Hangzhou
3. Department of Acupuncture and Moxibustion, First Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang Province.
- Publication Type:Journal Article
- Keywords:
bone cancer pain;
bone-nearby acupuncture;
electroacupuncture;
histone deacetylase 2 (HDAC2);
μ-opioid recepter (MOR);
histone deacetylase1 (HDAC1);
histone dorsal root ganglia;
morphine tolerance
- MeSH:
Acupuncture Points;
Animals;
Bone Neoplasms;
complications;
Cancer Pain;
therapy;
Drug Tolerance;
Electroacupuncture;
Ganglia, Spinal;
metabolism;
Histone Deacetylases;
metabolism;
Morphine;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Receptors, Opioid, mu;
metabolism
- From:
Chinese Acupuncture & Moxibustion
2020;40(4):405-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of early intervention of bone-nearby acupuncture (BNA) combined with electroacupuncture (EA) on the expression of histone deacetylase1(HDAC1), histone deacetylase 2 (HDAC2) andμ-opioid recepter (MOR) in dorsal root ganglia (DRG) of bone cancer pain-morphine tolerance (BCP-MT) rats, and to explore its possible mechanism.
METHODS:A total of 35 SD rats were randomized into a sham BCP group (=6), a BCP group (=7), a MT group (=7), a BNA+EA group (=8) and a shame BNA group (=7). Except of the sham BCP group, cancer cell inoculation operation at left tibia was given in the other 4 groups to establish the bone cancer pain model. In the MT group, the BNA+EA group and the shame BNA group, intraperitoneal injection of morphine hydrochloride was given to establish the morphine tolerance model. After the operation, bone-nearby acupuncture combined with electroacupuncture was applied at "Zusanli" (ST 36) and "Kunlun" (BL 60) in the BNA+EA group, with dilatational wave, 2 Hz/100 Hz in frequency, 0.5 to 1.5 mA in intensity. Intervention in the shame BNA group was applied at the same time and acupoints as those in the BNA+EA group, the needles were pierced the skin without any electrical stimulation. The needles were retained for 30 min, once a day for continuous 7 days in both BNA+EA and shame BNA groups. Before and 10, 11, 15, 22 days after the operation, the left paw withdrawal threshold (PWT) was measured in the 5 groups. The levels of HDAC1, HDAC2 and MOR in DRG were detected by Western blot.
RESULTS:Ten days after the cancer cell inoculation operation, the PWT of the BCP, MT, BNA+EA and sham BNA groups was decreased compared with the sham BCP group (<0.01). Eleven days after the operation, the PWT of the MT, BNA+EA and sham BNA groups was increased compared with the BCP group (<0.01). Twenty-two days after the operation, the difference was no significant between the BCP group and MT group (>0.05); the PWT of the BNA+EA group was increased compared with the MT and sham BNA group (<0.01). In the BCP group, the DRG levels of HDAC1 and HDCA2 were increased, while the level of MOR was decreased compared with the sham BCP group (<0.05, <0.01). In the MT group, the DRG level of HDAC1 was increased compared with the BCP group (<0.05). In the BNA+EA group, the DRG level of HDAC1 was decreased compared with the MT group and the sham BNA group (<0.01, <0.05), while the level of MOR was increased (<0.01).
CONCLUSION:Early intervention of bone-nearby acupuncture combined with electroacupuncture can relieve the morphine tolerance in bone cancer pain rats, it may relate to down-regulating the expression of HDAC1 and up-regulating the expression of MOR in the dorsal root ganglia.
