Gambogic acid enhances sensitivity of glioma U251 cells to temozolomide by inhibiting GLUT-3/ AKT signaling pathway
DOI：10.3872/j.issn.1007-385x.2020.08.007
- VernacularTitle:藤黄酸通过抑制GLUT-3/AKT信号通路增强神经胶质瘤U251细胞对替莫唑胺的敏感性
- Author:
LI Lei
1
;
LIU Rui
1
;
MIAO Cheng
1
Author Information
1. Department of Neurology, Xinxiang Central Hospital, Xinxiang 453000, Henan, China
- Publication Type:Journal Article
- Keywords:
glioma;
U251 cell;
gambogic acid;
temozolomide;
reactive oxygen species;
GLUT-3/AKT signaling pathway
- From:
Chinese Journal of Cancer Biotherapy
2020;27(8):879-883
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To explore whether gambogic acid can enhance the sensitivity of glioma U251 cells to temozolomide and further explore its mechanism. Methods: U251 cells were cultured in vitro and divided into blank control group, gambogic acid treatment group, temozolomide alone treatment group and combined treatment group. The cells survival rates of cells in each group was detected by CCK-8 assay. Flow cytometry was used to detect cell apoptosis and changes in ROS level. Western blotting was used to detect the changes in protein expressions. Results: CCK-8 results showed that the cells survival rates of the four groups after treatment for 24 h were (98.65±3.68)%, (93.58±2.47)%, (66.81±2.39)% and (38.65±4.13)%, respectively. It can be seen that the combined treatment could significantly increase the inhibitory effect of temozolomide on U251 cells. The proportion of apoptotic U251 cells in the combined treatment group was (61.43±2.58)%, which was significantly higher than that of (26.68±1.82)% in the temozolomide-treated group. Combined treatment of gambogic acid and temozolomide could up-regulate ROS level in U251 cells, reduce the expressions of GLUT-3 and p-AKT, and inhibit the GLUT-3/AKT signaling pathway. Conclusion: Gambogic acid combined with temozolomide can enhance the sensitivity of U251 cells to temozolomide by up-regulating ROS level and inhibiting GLUT-3/AKT signaling pathway in U251 cells, and provides a theoretical basis for the application of gambogic acid in the treatment of glioma.
- Full text:20200807.pdf
