Application of base editors in the treatment of genetic disorders
10.16438/j.0513-4870.2020-0942
- VernacularTitle:碱基编辑系统在遗传性疾病治疗中的应用
- Author:
Yuan CHEN
1
;
Yu-xuan CHEN
1
;
Yuan PING
1
Author Information
1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
- Publication Type:Research Article
- Keywords:
CRISPR/Cas9;
gene editing;
gene therapy;
base editor;
genetic disorder
- From:
Acta Pharmaceutica Sinica
2020;55(7):1562-1572
- CountryChina
- Language:Chinese
-
Abstract:
To date, CRISPR/Cas systems represent the most widely used tool for genome editing; however, its application scope for gene therapy has been largely limited due to its limited efficiency in activating homology-directed repair for DNA and off-target effect. Base editing is a new CRISPR/Cas-based genome-editing strategy, which allows single nucleotide to be precisely corrected in a narrow window scope on the target DNA or RNA by taking advantage of different nucleobase deaminases. Base editors include cytosine base editors (CBEs) and adenine base editors (ABEs), which can induce the conversions from C·G to T·A and A·T to G·C, respectively. Base editors work independently of double-strand DNA breaks (DSBs) and DNA donor templates, and thus they are extensively adopted for a wide range of therapeutic applications for genetic diseases, largely owing to their high efficiency and great specificity. In this review, we summarize the development of base editors and their potentials as therapeutic drugs for treating genetic diseases, and future outlooks are also discussed.
