Tumor microenvironment responsive biomimetic nanoparticles for photodynamic tumor therapy
10.16438/j.0513-4870.2020-0061
- VernacularTitle:肿瘤微环境响应的仿生金属纳米粒用于光动力学治疗的研究
- Author:
Rong-rong ZHENG
1
;
Lin-ping ZHAO
1
;
Hua-qing CHEN
1
;
Shi-ying LI
1
;
Xi-yong YU
1
Author Information
1. Guangdong Key Laboratory of Molecular Target and Clinical Pharmacology, State Key Laboratory of Respiratory Diseases Pharmacology Group, School of Pharmaceutical Sciences of Guangzhou Medical University, Guangzhou 511436, China
- Publication Type:Research Article
- Keywords:
histidine;
zinc;
nanomedicine;
coordination effect;
tumor microenvironment;
photodynamic therapy
- From:
Acta Pharmaceutica Sinica
2020;55(7):1672-1679
- CountryChina
- Language:Chinese
-
Abstract:
Inspired by the coordination effects between imidazole and metal ions in hemoglobin, biomimetic nanoparticles were constructed for photodynamic tumor therapy. The photosensitizer of protoporphyrin IX (PpIX) was modified with histidine, which could be self-assembled with Zn2+ to obtain the biomimetic nanoparticles (NPs). Under the conditions of high glutathione and low pH, the biomimetic nanoparticles could be degraded and released for enhanced photodynamic tumor therapy. The structures of NPs were characterized by dynamic light scattering (DLS), UV-visible spectrophotometer (UV-Vis), fluorescence microscope and transmission electron microscope (TEM). The reactive oxygen species (ROS) production ability of NPs was measured by singlet oxygen sensor green (SOSG) test kit. Mouse breast cancer cell lines (4T1 cells) were employed to investigate the subcellular organelle distribution and cytotoxicity of NPs. These results confirmed that NPs possessed a good dispersibility and stability with a uniform structure and particle size at 165 nm. Moreover, MTT assay and live/dead cell staining assay demonstrated that NPs could inhibit the proliferation of 4T1 cells and exhibit a good biocompatability. This research would promote the construction of intelligent biomedicine for tumor precision therapy.
