Effects of different immunogenic amino acids in PD-L1 vaccine on the differentiation of T cell subsets
10.11665/j.issn.1000-5048.20200313
- VernacularTitle:PD-L1疫苗中引入不同免疫原性氨基酸对T细胞亚群分化的影响
- Author:
Hongmei CHEN
1
;
Yanliang KANG
;
Li LIU
;
Wenbing YAO
;
Hong TIAN
Author Information
1. 中国药科大学生命科学与技术学院，江苏省生物药物成药性研究重点实验室
- Publication Type:Journal Article
- Keywords:
PD-L1;
vaccine;
3-nitrotyrosine;
4-nitrophenylalanine;
T cell differentiation
- From:
Journal of China Pharmaceutical University
2020;51(3):349-356
- CountryChina
- Language:Chinese
-
Abstract:
To compare the effects of endogenous 3-nitrotyrosine and non-natural 4-nitrophenylalanine in PD-L1 vaccine on the differentiation of T cell subsets, two immunogenic amino acids were introduced into the same site of PD-L1 vaccine. Two PD-L1 mutants with 3-nitrotyrosine and 4-nitrophenylalanine were obtained, respectively, using genetic code expansion technology. Mice were immunized with these two mutants, and their effects on the differentiation of T cell subsets in spleen were analyzed. The results of flow cytometry showed that the introduction of 4-nitrophenylalanine in PD-L1 vaccine could promote the polarization of Th1 cells while reducing the proportion of Treg cells; the introduction of 3-nitrotyrosine had no effect on the polarization of Th1 cells, while significantly increasing the proportion of Treg and Th17 cells. The introduction of both into PD-L1 vaccine could promote the response of CD8+ T cells in spleen, and the response of PD-L1 mutant containing 4-nitrophenylalanine was stronger. In summary, the non-natural 4-nitrophenylalanine is more suitable for the design of tumor vaccines as compared with endogenous 3-nitrotyrosine.
