CXCR4/SDF-1 axis regulates the effect of human lung adenocarcinoma PC-9 cells on function of in vitro blood-brain barrier model formed by Bends cells
10.3872/j.issn.1007-385x.2020.05.009
- VernacularTitle:CXCR4/SDF-1轴调节人肺腺癌PC-9细胞对Bends细胞体外血脑屏障模 型功能的影响
- Author:
LI Hongru
1
;
TU Xunwei
1
;
CHEN Zhengwei
1
;
CHEN Yusheng
1
;
HAN Lili
2
Author Information
1. (a. Department of Respiratory and Critical Medicine; b. Fujian Institute of Respiratory Diseases
2. c. Fujian Key Laboratory of Cardiovascular Diseases, Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University, Fuzhou 350001, Fujian, China
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
PC-9 cell;
blood brain barrier (BBB);
Bends cell;
tight junction;
transendothelial electric resistance (TEER);
migration;
CXCR4/SDF-1 axis
- From:
Chinese Journal of Cancer Biotherapy
2020;27(5):528-533
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the influences of human lung adenocarcinoma PC-9 cells on tight junction proteins of blood brain barrier (BBB) under CXCR4/SDF-1 axis by establishing a model of BBB in vitro. Methods: The immortalized mouse brain microvascular endothelial Bends cells were used to establish a model of BBB in vitro by monolayer culture; Subsequently, transendothelial electric resistance (TEER) and fluorescein sodium permeability experiment were used to detect the function of in vitro BBB model and observe the effect of PC-9 cells on the function of BBB model, respectively. Western blotting was used to detect the effect of PC-9 cells on function of BBB model and expressions of endothelial tight junction proteins under the treatment of single or combined AMD3100 and SDF-1 (1 μg/ml AMD3100,100 ng/ml SDF-1, AMD3100+SDF-1). Transwell assay was used to detect the influence of CXCR4/SDF-1 axis on the ability of PC-9 cells transmigrating the cell layer of BBB model. Results: Monolayer culture of Bends cells can form tightly connected BBB withhighTEER,which reached (182.13±5.19) Ω.cm2 at the 96 h; in the meanwhile, fluorescein sodium permeability experiment showed that BBB had significantly lower permeability than that of control group ([40.31±2.43]% vs [150.10±3.17]%, P<0.05). The TEER of BBB decreased to (46.7±4.35) Ω·cm2 after coculture with PC-9 cells for 24 h, and at the same time the sodium fluorescein permeability of BBB significantly increased than that of pre-treatment ([136.32±4.93]% vs [50.24±6.21]%, P<0.05). PC-9 cells up-regulated the expressions of tight junction proteins of Bends cells under the treatment of AMD3100 (P<0.05). The number of PC-9 cells transmigrating the BBB inAMD3100 treatment group was significantly lower than that of CON group (43±2 vs 81±2, P<0.05). Conclusion: AMD3100 can reduce the ability of PC-9 cells destroying the tight junction of the BBB model established in vitro by Bends cells.
- Full text:20200509.pdf
