Effect of Baicalin on myocardial cell apoptosis and Akt/AMPK/mTOR signal pathway expression in chronic myocardial failure rats
10.16571/j.cnki.1008-8199.2020.06.004
- VernacularTitle: 黄芩苷对慢性心肌衰竭大鼠心肌细胞凋亡及相关信号通路表达的影响
- Author:
Yu LIU
1
;
Ling GAO
1
Author Information
1. Deparment of Cardiology, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing 210008,Jiangsu,China
- Publication Type:Journal Article
- Keywords:
baicalin;
chronic myocardial failure;
cardiomyocyte apoptosis;
Akt;
AMPK;
mTOR
- From:
Journal of Medical Postgraduates
2020;33(6):577-581
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Baicalin on the apoptosis of cardiomyocytes and the expression of Akt/Amp activated protein kinase (AMPK)/mammalian rapamycin target protein (mTOR) signaling pathway in rats with chronic myocardial failure.MethodsSixty male SD rats of SPF grade were randomly divided into normal group, model group, positive control group and experimental group, with 15 rats in each group. Rats in the positive control group were given 40mg/kg losartan once a day. Rats in the experimental group were given 50mg/kg Baicalin once a day. Rats in the normal group and the model group were given the same dose of saline once a day. Each group was given gavage for 4 weeks. HE staining was used to observe the pathomorphology of cardiomyocytes. The myocardial tissue index (CAI) was detected by in situ end labeling. The expressions of Bax and bcl-2 protein in myocardium were detected by immunohistochemistry. The cardiac function was detected by echocardiography. The expression of Akt, AMPK and mTOR mRNA was detected by RT-PCR.ResultsCompared with the normal group, the apoptosis indexes of the model group, the positive control group and the experimental group were increased (P<0.05). Compared with the model group, the apoptosis indexes of the positive control group and the experimental group were decreased (P<0.05), while there was no significant difference between the positive control group and the experimental group (P>0.05). Compared with the model group, the expression of Bcl-2 protein was increased and Bax protein was decreased in the positive control group and the experimental group (P<0.05), while there was no significant difference between the positive control group and the experimental group (P>0.05). Compared with the normal group, the LVEFs of the model group, the positive control group and the experimental group were decreased, while the LVD and LVS were increased (P<0.05). Compared with the model group, the LVEFs of the positive control group and the experimental group were increased, while the LVD and LVS were decreased (P<0.05). The LVEF of the experimental group was higher than that of the control group, while the LVD and LVS of the experimental group were lower positive control group (P<0.05). Compared with the normal group, Akt, AMPK, mTOR mRNA expressions were decreased in the model group, positive control group and experimental group (P<0.05). Compared with the model group, Akt, AMPK, mTOR mRNA expressions were increased in the positive control group and experimental group (P<0.05), while there was no significant difference in Akt, AMPK, mTOR mRNA expression between the positive control group and experimental group (P>0.05).ConclusionBaicalin can alleviate myocardial injury in rats with chronic myocardial failure, and its mechanism may be related to the upregulation of Akt/AMPK/mTOR signaling pathway.
