DC-SIGN molecules promote EV71 infection in dendritic cells in vitro
10.13602/j.cnki.jcls.2019.04.08
- VernacularTitle:DC-SIGN受体促进肠道病毒71型体外感染树突状细胞
- Author:
Li ZHANG
1
;
Zhong XU
1
;
Donghua SHEN
1
;
Jianfeng ZHANG
1
Author Information
1. Department of Clinical Laboratory, Suzhou Hospital Affiliated to Nanjing Medical University
- Publication Type:Journal Article
- Keywords:
DC-SIGN may be one of the receptors of EV71 who promote EV71 infection in DCs and 293T cells in vitro
- From:
Chinese Journal of Clinical Laboratory Science
2019;37(4):274-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of dendritic cell specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expression on the infection of enterovirus type 71 (EV71) for dendritic cells (DCs) and 293T cells.
Methods:Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood by gradient density centrifugation and induced to DCs. The DC-SIGN gene was amplified by polymerase chain reaction (PCR) for construction of transient expression vector pLB-DC-SIGN which was then transfected into 293T cells using Lipofectamine TM 2000. After 293T cell and 293T-pLB-DC-SIGN was infected with EV71, EV71 mRNA was identified by fluorescence quantitative PCR. EV71/VP1 was identified by western blot. After the expression of DC-SIGN on DCs was blocked by yeast mannan, EV71 mRNA was identified by fluorescence quantitative PCR and EV71/VP1 was identified by western blot.
Results:EV71 viral load and EV71/VP1 expression level in 293T cells overexpressed DC-SIGN was significantly higher than that of control 293T, while the viral load and EV71/VP1 expression level in DCs blocked with DC-SIGN inhibitor was lower than that of control DCs (P＜0.05).
Conclusion:DC-SIGN may be one of the receptors of EV71 who promote EV71 infection in DCs and 293T cells in vitro.
