Emodin assay in Ampelopsis radix by RP-HPLC
10.12206/j.issn.1006-0111.201911114
- VernacularTitle:白蔹药材中大黄素含量的反相高效液相色谱法测定
- Author:
Fei MENG
1
;
Xueli SONG
1
Author Information
1. Zibo Branch of No. 960 Hospital of Joint Logistic Support Force of PLA, Zibo 255300, China.
- Keywords:
Ampelopsis radix;
emodin;
HPLC
- From:
Journal of Pharmaceutical Practice
2020;38(3):264-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for Emodin assay in Ampelopsis radix, and to improve the quality control of Ampelopsis radix. Methods Reversed phase high performance liquid chromatography was used. The column: Apollo-C18 (4.6 mm×250 mm, 5 μm); mobile phase: methanol:0.2% phosphoric acid (85:15); flow rate: 1.0 ml/min; column temperature: room temperature; detection wavelength: 220 nm. Results With the specified chromatographic conditions, there was a good linear relationship for Emodin in Ampelopsis radix in the range of 0.124-3.968 μg/ml. The linear regression equation was Y= 53 962X–966.46, r=0.999 7. The average recovery was 99.7%. RSD=2.5% (n=9). Conclusion This method is accurate, sensitive and repeatable. It is suitable for the determination of emodin in Ampelopsis radix.
