Mechanism of Xihuang extract in inhibiting proliferation of gastric cancer SGC-7901 cells
10.3872/j.issn.1007-385x.2018.05.008
- VernacularTitle:西黄浸提液抑制胃癌SGC-7901细胞增殖的可能机制
- Author:
TANG Yuan
1
;
LIANG Chao
1
;
CAI Qingping
1
Author Information
1. (Department of Gastro-intestine Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
- Publication Type:Journal Article
- Keywords:
Xihuang extract;
gastric cancer;
SGC7901 cell;
proliferation;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(5):485-489
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of Xihuang (XH) extract on the proliferation of gastric cancer SGC-7901 cells and its underlying mechanism. Methods: Gastric cancer cell line SGC-7901 was conventionally cultured. CCK-8 assay and flow cytometry were used to detect the effect of different concentrations of XH extracts (3.2, 6.4, 12.8, and 25.6 mg/ml) on proliferation and apoptosis of SGC-7901 cells after treatment for different time periods (24, 48, and 72 h); The effect of different concentrations of XH extracts on the mRNA expression of apoptosis-related genes (Bax and Bcl-2) was detected by qPCR; Western blotting was used to detect the effect of XH extracts on the expression of apoptosis-associated proteins (caspase 3, caspase 9, Bax and Bcl-2). Results: XH extracts (3.2, 6.4, 12.8, and 25.6 mg/ml) could effectively inhibit proliferation of gastric cancer SGC-7901 cells (P<0.05 or P<0.01) in a concentration-depend manner (P<0.01). XH extract could significantly up-regulate Bax mRNAand down-regulate Bcl-2 mRNAexpression (P<0.05 or P <0.01); Meanwhile, XH extract ouldincrease protein expressions of caspase 3, caspase 9, Bax but reduce Bcl-2 protein expression (P< 0.05 or P<0.01). Conclusion: XH extract can inhibit the proliferation of gastric cancer SGC-7901 cells by triggering apoptosis, which may become a potential method of adjuvant treatment of gastric cancer.
- Full text:20180508.pdf
