Alpha-adrenergic receptors in PVT neurons promote recovery from propofol anesthesia
10.16571/j.cnki.1008-8199.2019.09.005
- VernacularTitle: 肾上腺素能α受体在丙泊酚麻醉觉醒中的作用
- Author:
Jun-xiao LIU
1
;
Cheng-xi LIU
1
;
Ping LÜ
1
;
Bao FU
2
Author Information
1. The First Clinical Institute, Zunyi Medical University, Zunyi 56300, Guizhou, China
2. Department of Critical Care Medicine,Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou, China
- Publication Type:Journal Article
- Keywords:
propofol;
paraventricular nucleus;
alpha-adrenergic receptors;
c-fos;
emergence time
- From:
Journal of Medical Postgraduates
2019;32(9):920-925
- CountryChina
- Language:Chinese
-
Abstract:
Objective The locus coeruleus noradrenergic system regulates the recovery process of general anesthesia, but its mechanism remains unclear. The locus coeruleus has a large amount of projection to the paraventricular nucleus of the thalamus (PVT). This study was to investigate the effect of the α-noradrenergic receptor in PVT neurons in propofol anesthesia. Methods The immunofluorescence technique was used for comparison of the c-fos expression in the PVT neurons collected from male SD rats under propofol anesthesia (the PA group, n = 4) or no anesthesia (the non-PA group, n = 4) and observation of the activity of PVT neurons. PVT microinjection models were established in 40 rats and randomized into four groups of equal number: noradrenaline, phentolamine, propranolol, and isotonic saline. Under propofol anesthesia, the animals received microinjection of noradrenaline, phentolamine, propranolol, and isotonic saline at 1 μL into the PVT, respectively, and were observed for the time of recovery of righting reflex (RORR) and the δ (1-4 Hz), θ (4-8 Hz), α (8-12 Hz), β (12-25 Hz) and γ waves (25-60 Hz) on EEG before and after microinjection. Results The expression of c-fos was significantly reduced in the PA group compared with that in the non-PA control. The Ca2+ signals in the PVT were significantly increased during the propofol induction of the loss of righting reflex (LORR), but decreased in the early stage of and during propofol anesthesia (P < 0.05), and remarkably increased at the emergence of and during RORR (P < 0.05). In comparison with the isotonic saline control, the noradrenaline group showed markedly shortened time of RORR (837.8 s vs 647.7 s, P < 0.05), reduced rate of δ waves (P < 0.05) and elevated rate of β waves (P < 0.05), while the phentolamine group exhibited prolonged time of RORR (837.8 s vs 1045.1 s, P < 0.05) and increased rate of δ waves after microinjection (P < 0.05). Conclusion The α-noradrenergic receptors in PVT neurons play a critical role in promoting recovery from propofol anesthesia.
