Drug resistance and molecular characteration of hemagglutinin and neuraminidase for influenza A H3N2 viruses
10.16571/j.cnki.1008-8199.2019.12.005
- VernacularTitle: 2017年江苏省H3N2流感病毒血凝素与神经氨酸酶分子特征及耐药性分析
- Author:
Shen-jiao WANG
1
;
Xian QI
1
;
Fei DENG
1
;
Jie-fu PENG
1
;
Hui-yan YU
1
;
Yuan-fang QIN
1
;
Qi-gang DAI
1
;
Chang-jun BAO
1
Author Information
1. Department of Acute Infectious Disease, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, Jiangsu,China
- Publication Type:Journal Article
- Keywords:
influenza virus;
influenza A virus subtype H3N2;
HA;
NA;
genetic characteristics;
drug resistance analysis
- From:
Journal of Medical Postgraduates
2019;32(12):1254-1258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the variations and drug resistance of influenza A (H3N2) viruses in Jiangsu Province in 2017, and provide evidence for prevention and control strategies on influenza. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used for the sequencing of H3N2 subtype influenza strains. The influenza reference sequences were obtained from the global shared influenza site GISAID. The sequence alignment and phylogenetic analysis were performed using MAGE7.0 software. Viral resistance was analyzed by a neuraminidase inhibition assay. Results The H3N2 subtype influenza isolates and vaccine strains belonged to the 3C.2a branch of the H3 subtype. Some of the strains showed amino acid mutations on the immune-related sites named N121K, T135K and N171K. The isolates were sensitive to the flu drugs oseltamivir and zanamivir. Conclusion The H3N2 epidemic strains in Jiangsu have genetic recombination within subtypes and are still sensitive to neuraminidase inhibitors. As the H3N2 influenza virus mutations continue, a close monitoring of the viral genetic evolution and the drug resistant genes should be guaranteed.
