Simultaneous Determination of 7 Anti-rheumatic Active Ingredients in Mongolian Medicine Sendeng- 4 Decoction Powder by HPLC-DAD
- VernacularTitle:HPLC-DAD法同时测定蒙药森登-4汤散中7种抗风湿活性成分的含量
- Author:
SURITALATU
1
;
Jiaqi YU
2
;
Xiye WANG
2
;
Jinglin LIU
2
;
Ning XU
2
;
Liang XU
2
Author Information
1. Dept. of Mongolian Medicine Preparation，the Affiliated Hospital of Inner Mongolia University for the Nationalities，Inner Mongolia Tongliao 028042，China
2. College of Chemistry and Chemical Engineering/Inner Mongolia Key Laboratory of Natural Products Chemistry and Functional Molecules Synthesis，Inner Mongolia University for Nationalities，Inner Mongolia To ngliao 028042，China
- Publication Type:Journal Article
- Keywords:
Sendeng-4 decoction powder;
HPLC-DAD;
Catechin;
Dihydromyricetin;
Texifolin;
Jasminoidin;
Rutin;
Myricetin;
Quercetin;
Content
- From:
China Pharmacy
2019;30(2):221-225
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder， such as catechin， jasminoidin， dihydromyricetin， texifolin， rutin， myricetin and quercetin. METHODS： HPLC-DAD method was adopted. The determination was performed on Sil Green C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1 mL/min. The detection wavelength was set at 238 nm， and column temperature was 30 ℃. The sample size was 10 μL. RESULTS： The linear range of catechin， jasminoidin， dihydromyricetin， texifolin， rutin， myricetin and quercetin were 8.590-2 290， 10.56-3 901， 13.00-3 958， 8.815-564.2， 4.030-257.8， 8.130-750.5， 7.075-454.2 μg/mL （r≥0.999 1）， respectively； the limits of detection were 0.429 5， 0.264 0， 0.325 0， 0.220 4， 0.201 5， 0.203 2， 0.176 9 μg/mL， respectively； the limits of quantification were 1.030， 1.321， 1.302， 1.397， 1.637， 0.813 0， 0.707 5 μg/mL， respectively. RSDs of precision， stability （48 h） and repetition tests were all lower than 2.0％ （n＝6）. The average recoveries were 96.24％-99.28％ （RSD＝1.03％-1.63％， n＝6）. CONCLUSIONS： The established method is simple， accurate and reproducible， and can be used for simultaneous determination of catechin， jasminoidin， dihydromyricetin， texifolin， rutin， myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.
