Effect of stress on myocardial apoptosis in ischemic preconditioning in rabbit hearts.
10.3969/j.issn.1672-7347.2014.05.007
- Author:
Huashan XU
1
;
Shengxi CHEN
;
Wanjun LUO
;
Khan Mohammed FIROJ
Author Information
1. Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052; Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Etomidate;
pharmacology;
Heart;
drug effects;
Ischemic Preconditioning;
Ischemic Preconditioning, Myocardial;
Methylprednisolone;
pharmacology;
Myocardium;
pathology;
Rabbits
- From:
Journal of Central South University(Medical Sciences)
2014;39(5):477-482
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the influence of stress on myocardial apoptosis in ischemic preconditioning group (IPC).
METHODS:Twenty-four Japanese white rabbits were randomly divided into 4 groups (n=6): an etomidate group (the Etom group) of depressed stress established by intravenous etomidate, an IPC group, an ischemic reperfusion group (the IR group) and a methylprednisolone group (the MP group). Myocardial apoptosis was examined by DNA-laddering, in situ nick-end labeling (TUNEL) and Hoechst dyeing.
RESULTS:The DNA ladder increased in the Etom group. The percentage of apoptosis by TUNEL method was 1.7%±0.2% in the IPC group, 2.3%±0.8% in the MP group, 3.8%±1.3% in the IR group and 3.0%±0.4% in the Etom group. Hoechst dying was 4.1%±0.9% in the IPC group, 3.5%±0.4% in the MP group, 6.2%±1.6% in the IR group and 7.6%±0.4% in the Etom group. There was significant difference between the IPC group and the Etom group or IR group, and also between the MP group and the IR group.
CONCLUSION:A depressed stress response impairs the inhibition on myocardial apoptosis in ischemic preconditioning. Methylprednisolone may inhibit myocardial apoptosis.
