miR-214 inhibits the osteogenic differentiation of dental follicle cells in vitro
10.12016/j.issn.2096-1456.2020.03.002
- Author:
WANG Zhiheng
1
;
ZUO Jie
1
;
WANG Mengqi
1
;
ZHU Shaojun
1
;
LIU Yishan
1
Author Information
1. Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology
- Publication Type:Journal Article
- Keywords:
dental follicle cells;
bidirectional differential passage;
miR-214;
Wnt/β-catenin;
osteogenic differentiation;
alkaline phosphatase;
osteonectin;
mineralized nodule
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(3):146-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect of miR-214 on the osteogenic differentiation of dental follicle cells (DFCs).
Methods:Purified DFCs were cultured in vitro by bidirectional differential passage, with the untransfected DFCs as the control group (DFCs group). The expression of miR-214-3p in DFCs was upregulated and downregulated by transfection of miR-214-3p(miR-214 mimics group) or miR-214-3p inhibitors(miR-214 inhibitor group) into DFCs. The expression levels of miR-214, alkaline phosphatase (ALP), osteonectin (OSN) and runt-related transcription factor-2(RUNX-2) were detected by qRT-PCR after 7 days of osteogenesis induction, the protein expression levels of RUNX-2 and β-catenin were detected by western blot, and the formation of mineralized nodules was observed with alizarin red staining after 14 days of osteogenesis induction.
Results : Compared with the DFCs group, in the miR-214 mimics group, the expression of miR-214 was upregulated after 7 days of osteogenesis induction. The mRNA expression of ALP, OSN and RUNX-2 in the miR-214 mimics group was lower than that in the DFCs group, but only ALP in the two groups was statistically significant (P > 0.05); the mRNA expression of ALP, OSN and RUNX-2 in the miR-214 inhibitor group was higher than that in the DFCs group, and the difference was statistically significant (P < 0.05). The protein expression of RUNX-2 and β-catenin in the miR-214 mimics group was lower than that in the miR-214 inhibitor group. The number of calcified nodules in the miR-214 mimics group was significantly less than that in the DFCs group, while that in the miR-214 inhibitor group was significantly higher than that in the DFCs group.
Conclusion:The upregulation of miR-214 can downregulate the expression of β-catenin, can inhibit the expression of ALP, OSN and RUNX-2 related to osteogenesis, and can inhibit osteogenic differentiation. The downregulation of miR-214 demonstrated the opposite results; miR-214 may downregulate the expression of β-catenin and inhibit the osteogenic differentiation of DFCs.
- Full text:miR-214抑制牙囊细胞成骨分化的体外研究.pdf
