Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats.
10.11817/j.issn.1672-7347.2015.02.005
- Author:
Chenggui MIAO
1
,
2
;
Guoliang ZHOU
;
Meisong QIN
;
Jianzhong CHEN
;
Chengfeng LI
;
Huaqi HE
Author Information
1. Department of Pharmaceutics, Food and Drug College, Anhui Science and Technology University , Fengyang Anhui 233100; Department of Basic and Clinical Pharmacology, College of Pharmacy, Anhui Medical University, Heifei 230032; Poultry Disease Monitoring Anhui Key Laboratory, Anhui Science and Technology University, Fengyang Anhui 233100, China fensheng1981@
2. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Arthritis, Experimental;
Caspase 3;
metabolism;
Fibroblasts;
cytology;
drug effects;
Interleukin-6;
metabolism;
Interleukin-8;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Pulsatilla;
chemistry;
Rats;
Rats, Sprague-Dawley;
Synovial Membrane;
cytology;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2015;40(2):144-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
METHODS:A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
RESULTS:Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
CONCLUSION:PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.
