Interaction between functional nano-hydroxyapatite and cells and the underlying mechanisms.
10.11817/j.issn.1672-7347.2016.09.008
- Author:
Min YANG
1
;
Yanzhong ZHAO
2
;
Guohui WANG
1
;
Juan TAN
1
;
Shaihong ZHU
2
Author Information
1. Medical Experimental Center, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Medical Experimental Center, Third Xiangya Hospital; Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Arginine;
pharmacology;
Biological Transport, Active;
physiology;
Caveolins;
physiology;
Cells, Cultured;
Clathrin;
physiology;
Durapatite;
pharmacokinetics;
Endocytosis;
physiology;
Human Umbilical Vein Endothelial Cells;
cytology;
Humans;
Nanoparticles;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2016;41(9):937-945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the interaction between arginine functionalized hydroxyapatite (HAP/Arg) nanoparticles and endothelial cells, and to investigate mechanisms for endocytosis kinetics and endocytosis.
METHODS:Human umbilical vein endothelial cells (HUVECs) were selected as the research model.Cellular uptake of HAP/Arg nanoparticles were observed by laser scanning confocal microscopy.Average fluorescence intensity of cells after ingestion with different concentrations of HAP/Arg nanoparticles were determined by flow cytometer and atomic force microscopy.
RESULTS:The HAP/Arg nanoparticles with doped terbium existed in cytoplasm, and most of them distributed around the nucleus area after cellular uptake by HUVECs. Cellular uptake process of HAP/Arg nanoparticles in HUVECs was in a time and concentration dependent manner. 4 h and 50 mg/L was the best condition for uptake. HAP/Arg nanoparticles were easier to be up-taken into the cells than HAP nanoparticles without arginine functionalized.
CONCLUSION:HAP/Arg nanoparticles are internalized by HUVECs cells through an active transport and energy-dependent endocytosis process, and it is up-taken by cells mainly through caveolin-mediated endocytosis, but the clathrin-dependent endocytic pathway is also involved..
