Impact of deep slow-wave sleep deprivation on oxidative stress response of the testis tissue in rats.
- Author:
Fei XU
1
;
Na YANG
2
;
Yu-Han LIANG
3
;
Qi-Jin HE
3
;
Mei-Wen ZHANG
3
;
Zi-Han WANG
3
;
Yi-Wei SHEN
3
;
Hai-Zheng LI
3
;
Xue-Qian WANG
3
;
Lai-Xiang LIN
1
Author Information
1. Research Institute of Endocrinology / Key Laboratory of Hormones and Development / Key Laboratory for Metabolic Diseases, Hospital of Metabolic Diseases, Tianjin Medical University, Tianjin 300070, China.
2. The Second School of Clinical Medicine, Tianjin Medical University, Tianjin 300070, China.
3. School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
- Publication Type:Journal Article
- Keywords:
flowerpot technique;
oxidative stress;
rat;
sleep deprivation;
testis
- MeSH:
Animals;
Body Weight;
Glutathione Peroxidase;
analysis;
Male;
Malondialdehyde;
Oxidative Stress;
Random Allocation;
Rats;
Rats, Wistar;
Sleep Deprivation;
metabolism;
Sleep Stages;
Superoxide Dismutase;
analysis;
Testis;
metabolism;
pathology;
Time Factors;
Weight Loss
- From:
National Journal of Andrology
2017;23(8):687-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of deep slow-wave sleep deprivation on the oxidative stress of testicular tissue in rats.
METHODS:Thirty-six 5-week-old male Wistar rats were equally randomized into deep slow-wave sleep deprivation group (SD1), deep slow-wave sleep and duration sleep deprivation group ( SD2), and a cage control group (CC). The rat model of deep slow-wave sleep deprivation was established using the flowerpot technique. The rats in the SD1 group were interfered every 24 minutes and deprived of 12 hours of sleep at night, those in the SD2 group deprived of 8 minutes of sleep at an interval of 24 minutes and 12 hours of sleep at night, and those in the CC group exposed to 12 hours of daylight and 12 hours of darkness. After 28 days, all the rats were executed for measurement of the testis volume and protein content, determination of the methane dicarboxylic aldehyde (MDA) level and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and observation of the pathological changes in the testicular tissue under the microscope.
RESULTS:Compared with the CC group, the rats in the SD1 and SD2 groups showed significantly reduced body weight (［268.5 ± 1.6］ vs ［248.1 ± 25.1］and［232.9 ± 10.1］g, P<0.05) and increased relative testis mass (［50.0 ± 1.3］ vs ［57.9 ± 6.1］ and ［54.9 ± 3.5］ ×10⁻², P<0.05). Statistically significant differences were found between the CC and SD2 groups in the contents of protein (［6.3 ± 1.4］ vs ［4.5 ± 0.9］ gpro/L, P<0.05) and MDA (［1.1 ± 0.1］ vs ［1.3 ± 0.3］ nmol/mgpro, P<0.05) and the activities of SOD (［104.3 ± 33.1］ vs ［135.2 ± 26.9］ U/mgpro, P<0.05) and GSH-Px (［15.6 ± 4.0］ vs ［21.7 ± 4.3］ U/mgpro, P<0.05), but not between the CC and SD1 groups (P>0.05). The lumens in the testis were narrowed, with obvious hyperplasia, hyperemia and edema in the peripheral interstitial tissue, but no significant pathologic changes were observed in the testis tissue of the SD1 group.
CONCLUSIONS:Long-term deprivation of deep slow-wave sleep impairs the structure of the testis tissue and induces oxidative stress response in rats.
