Excessive fluoride increases the expression of osteocalcin in the mouse testis.
- Author:
Jian-Hua QIN
1
;
Aierken DILINUER
1
;
Shataer SAIMIRE
1
;
Aierken KALIBINUER
1
;
Mahemuti YUSUFU
1
;
Aili YIRIZHATI
1
;
Sai-Sai CUI
1
;
Maimaitiming NUERSIMANGULI
1
;
Wu-Jin CHEN
1
;
Sheng-Bin BAI
1
Author Information
1. School of Preclinical Medicine, Xinjiang Medical University, Urumqi, Xinjiang 830011, China.
- Publication Type:Journal Article
- Keywords:
mouse;
osteocalcin;
testis;
testosterone;
fluoride
- MeSH:
Animals;
Fluoride Poisoning;
metabolism;
Fluorides;
toxicity;
Male;
Mice;
Mice, Inbred C57BL;
Osteocalcin;
metabolism;
Random Allocation;
Sodium Fluoride;
toxicity;
Testis;
drug effects;
metabolism
- From:
National Journal of Andrology
2017;23(9):782-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influence of excessive fluoride on the levels of osteocalcin and testosterone in the testis of the male mouse.
METHODS:Twenty-four C57BL/6J male mice were equally randomized into a normal control and a fluorosis model group, the former fed on distilled water while the latter on a solution of sodium fluoride (100 mg/L) in distilled water, both for 12 weeks. Then, the level of osteocalcin in the testis tissue was measured with the immunohistochemical streptavidin-peroxidase (SP) method and those of osteocalcin and testosterone in the serum determined by ELISA.
RESULTS:After 12 weeks of fluoride intervention, the level of serum osteocalcin was significantly higher in the fluorosis models than in the normal controls (［68.05 ± 5.32］ vs ［47.50 ± 5.73］ pg/mL, F = 11.901, P = 0.008), while that of testosterone markedly lower in the former than the latter group (［8.07 ± 1.35］ vs ［12.94 ± 3.09］ ng/mL, F = 2.313, P = 0.006). The results of immunohistochemical SP showed the expression of osteocalcin in the cell membrane and cytoplasm of the fluorosis models, which was evidently higher than in the normal controls.
CONCLUSIONS:Twelve-week intake of 100 mg/L fluoride solution can decrease the level of testosterone and increase the expression of osteocalcin in the testis of the male mouse.
