Effects of boiling duration in processing of White Paeony Root on its overall quality evaluated by ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics analysis and high performance liquid chromatography quantification.
10.1016/S1875-5364(17)30009-2
- Author:
Kong MING
1
;
Jun XU
2
;
Huan-Huan LIU
1
;
Jin-Di XU
1
;
Xiu-Yang LI
1
;
Min LU
1
;
Chun-Ru WANG
1
;
Hu-Biao CHEN
3
;
Song-Lin LI
4
,
5
Author Information
1. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Sciences and Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
2. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China.
3. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China. Electronic address: hbchen@hkbu.edu.hk.
4. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Sciences and Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China. Electronic address: songlinli64@
5. com.
- Publication Type:Journal Article
- Keywords:
Boiling processing;
Major bioactive components;
Metabolomics;
Overall quality;
White Paeony Root
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
standards;
Hot Temperature;
Mass Spectrometry;
methods;
Paeonia;
chemistry;
Plant Roots;
chemistry;
Technology, Pharmaceutical;
Water
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(1):62-70
- CountryChina
- Language:English
-
Abstract:
Boiling processing is commonly used in post-harvest handling of White Paeony Root (WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling (2-10 min) is recommended for post-harvest handling of WPR.
