Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng.
10.1016/S1875-5364(14)60006-6
- Author:
Qian MAO
1
;
Yi LI
2
;
Song-Lin LI
1
;
Jie YANG
3
;
Ping-Hu ZHANG
4
,
5
;
Qiang WANG
5
,
6
Author Information
1. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Science, Nanjing 210009, China.
2. Nanjing Normal University Center for Analysis and Testing, Nanjing Normal University, Nanjing 210009, China.
3. State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China.
4. Jiangsu Center for New Drug Screening & National New Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210009, China. Electronic address: zhangpinghu@
5. com.
6. State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: qwang49@
- Publication Type:Journal Article
- Keywords:
Chemical profile;
Cytotoxic effects;
Leaf material;
Panax notoginseng;
R284;
R285;
Saponin
- MeSH:
Antineoplastic Agents, Phytogenic;
chemistry;
pharmacology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Humans;
Mass Spectrometry;
Panax notoginseng;
chemistry;
Plant Leaves;
chemistry;
Saponins;
chemistry;
pharmacology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(1):30-37
- CountryChina
- Language:English
-
Abstract:
AIM:To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction (TSF), 25% ethanol fraction (25EF), 50% ethanol fraction (50EF), and 85% ethanol fraction (85EF) prepared by macroporous resin from the leaves of Panax notoginseng.
METHOD:The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MS(n) analysis. The cytotoxic effects were determined against KP4 cells (human pancreatic cancer), NCI-H727 cells (human lung cancer), HepG2 cells (human hepatocellular cancer), and SGC-7901 cells (human gastric adenocarcinoma).
RESULTS:Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity.
CONCLUSION:From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.
