Transformation of astragalosides from radix astragali under acidic, neutral, and alkaline extraction conditions monitored by LC-ESI-TOF/MS.
10.1016/S1875-5364(14)60062-5
- Author:
Chu CHU
1
;
E-Hu LIU
1
;
Lian-Wen QI
2
,
3
;
Ping LI
3
,
4
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: fleude@
3. com.
4. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liping2004@
- Publication Type:Journal Article
- Keywords:
Astragalosides;
LC-TOF/MS;
Radix Astragali;
Transformation
- MeSH:
Astragalus Plant;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Hydrogen-Ion Concentration;
Molecular Structure;
Plant Roots;
chemistry;
Tandem Mass Spectrometry;
Triterpenes;
chemistry;
isolation & purification
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(4):314-320
- CountryChina
- Language:English
-
Abstract:
AIM:To reveal the profile of astragalosides for better quality evaluation of Radix Astragali, this study was aimed to investigate the transformation of astragalosides under different conditions.
METHOD:Seven major astragalosides were selected for evaluation under acidic, neutral and alkaline conditions. The transformation in real plant samples was also examined and the products were characterized by LC-ESI-TOF/MS.
RESULTS:In weak acidic solution, all of the astragalosides are stable. In addition, the transformation ratios of the astragalosides under neutral and alkaline conditions were also obtained.
CONCLUSION:In neutral solution, malonylastragaloside I was transformed to astragaloside I; and in alkaline solution, substituent group(s) in the xylose moiety of all the astragalosides were eliminated. Since astragalosdie IV is the basic skeleton structure of the astrgalosides, it is a common transformation product of other astragalosides.
