Influence of ultrafiltration membrane on ophiopogonins and homoisoflavonoids in Ophiopogon japonicus as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry.
10.1016/S1875-5364(17)30028-6
- Author:
Chun-Hua LIU
1
;
Jin QI
2
;
Da-Zheng ZHOU
3
;
Ai-Chun JU
4
;
Bo-Yang YU
5
,
6
Author Information
1. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.
2. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China.
3. Department of Technology Development, TianJin Tasly Pride Pharmaceutical Co., Ltd., Tianjin, 300410, China.
4. Department of Technology Development, TianJin Tasly Pride Pharmaceutical Co., Ltd., Tianjin, 300410, China. Electronic address: juach@tasly.com.
5. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@
6. com.
- Publication Type:Journal Article
- Keywords:
Homoisoflavonoids;
Ophiopogon japonicus (L.f) Ker-Gawl.;
Ophiopogonins;
UFLC-IT-TOF/MS;
Ultrafiltration
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Chromatography, Liquid;
methods;
Drugs, Chinese Herbal;
Isoflavones;
analysis;
Molecular Structure;
Molecular Weight;
Ophiopogon;
chemistry;
Plant Extracts;
chemistry;
Polymers;
Saponins;
analysis;
Spectrometry, Mass, Electrospray Ionization;
methods;
Sulfones;
Ultrafiltration;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(2):121-141
- CountryChina
- Language:English
-
Abstract:
Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus (L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off (MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone (PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate (%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
