Quantitation of camellianin A in HepG2 cells using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method.
10.1016/S1875-5364(17)30040-7
- Author:
Qian-Qian CHEN
1
;
Hai-Ling XI
2
;
Cai-Yun WANG
1
;
Feng-Guo XU
3
;
Wei ZHANG
4
Author Information
1. State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China.
2. School of Public Health, Baotou Medical College, Baotou 014060, China.
3. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China.
4. State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. Electronic address: wzhang@must.edu.mo.
- Publication Type:Journal Article
- Keywords:
Camellianin A;
HPLC-MS/MS;
HepG2 cells
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Flavonoids;
chemistry;
Hep G2 Cells;
Humans;
Molecular Structure;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(3):234-240
- CountryChina
- Language:English
-
Abstract:
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol-water (0.1% formic acid) (55 : 45, V/V). The total run time was 5.0 min. The method was linear in the concentration range of 0.25-250.0 ng·mL. The lower limit of quantification was 0.25 ng·mL. The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
