Identification of C-geranylated flavonoids from Paulownia catalpifolia Gong Tong fruits by HPLC-DAD-ESI-MS/MS and their anti-aging effects on 2BS cells induced by HO.
10.1016/S1875-5364(17)30059-6
- Author:
Wen-Zhao TANG
1
;
Ying-Ai WANG
2
;
Tian-Yang GAO
2
;
Xiao-Jing WANG
2
;
Yun-Xue ZHAO
3
Author Information
1. Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Jinan 250062, China; State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: twzsd@sina.com.
2. Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Biotech-Drugs Ministry of Health, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Jinan 250062, China.
3. Department of Pharmacology, School of Medicine, Shandong University, Jinan 250012, China.
- Publication Type:Journal Article
- Keywords:
2BS cells;
Anti-aging effects;
C-geranylated flavonoids;
HPLC-DAD-ESI-MS/MS;
Paucatalinone C;
Paulownia catalpifolia Gong Tong
- MeSH:
Aging;
drug effects;
Cell Line;
Chromatography, High Pressure Liquid;
Flavonoids;
chemistry;
isolation & purification;
pharmacology;
Fruit;
chemistry;
Humans;
Hydrogen Peroxide;
toxicity;
Magnoliopsida;
chemistry;
Molecular Structure;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology;
Tandem Mass Spectrometry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(5):384-391
- CountryChina
- Language:English
-
Abstract:
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C (1) and five known C-geranylated flavanones (2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CHCl extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by HO. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone (3) and paucatalinone A (5) were the potent anti-aging agents in the premature senescent 2BS cells induced by HO and the C-geranyl substituent may be an important factor because of its lipophilic character.
