Gadoxetic disodium-enhanced MRI to characterize T1 relaxation values and expression level of organic anion transporters and multidrug resistance protein on hepatocyte surface membrane of normal C57BL/6 mice
10.3760/cma.j.issn.1007-3418.2019.07.013
- VernacularTitle: 正常C57BL/6小鼠钆塞酸二钠增强磁共振检查T1弛豫值特征及肝细胞膜表面有机阴离子转运系统和多耐药蛋白载体的表达水平
- Author:
Ying DING
1
;
Li YANG
;
Shengxiang RAO
;
Mengsu ZENG
Author Information
1. Department of Radiology, Zhongshan Hospital, Fudan University; Shanghai Institute of Medical Imaging; Department of Medical Imaging, Shanghai Medical College, Fudan University, Shanghai 200032, China
- Publication Type:Journal Article
- Keywords:
Magnetic resonance imaging;
Gadoxetic acid;
T1 mapping;
C57BL/6 mice;
Organic anion transporter;
Multi-drug resistance protein
- From:
Chinese Journal of Hepatology
2019;27(7):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The characteristics of T1 relaxation values and the expression levels of organic anion transport system (OATP) and multidrug resistance protein carrier (MRP) on hepatocyte surface membrane were quantitatively studied to evaluate liver function in normal C57BL/6 mice with gadoxetic disodium-enhanced MRI.
Methods:Ten 6-weeks-old, normal C57BL/6 mice were included in this study. Gadoxetic disodium- enhanced MRI examination was performed. Longitudinal relaxation time images before and 20 min after contrast injection (hepatobiliary-specific phase) were acquired. T1-relaxation time, T1 relaxation time decline rate (△T) and rapid initial enhancement slope percentage in the first-pass study of the liver parenchyma before and after administration of gadoxetate disodium were measured. Liver parenchyma specimens were detected by Western blotting and the values of OATP1, MRP2, and MRP3 were recorded. Statistical results were expressed in mean.
Results:The mean T1 relaxation time of 10 normal C57BL/6 mice before and after enhancement was 659.13 ± 24.07, and 408.87 ± 27.21 ms. The mean T1 relaxation time decline rate and rapid initial enhancement slope percentage in the first-pass study was 37.12% ± 4.95% and 4.14% ± 0.96% ms. Furthermore, the mean value of OATP1, MRP2 and MRP3 were 29 952.1 ± 11 475.2, 34 376.4 ± 33 228.4 and 357 308.9 ± 64 646.5.
Conclusion:T1-relaxation values, T1 relaxation time decline rate and rapid initial enhancement slope percentage in the first-pass study before and after gadoxetic disodium-enhanced MRI were determined in normal C57BL/6 mice as well as quantitative values of OATP1, MRP2 and MRP3 at the molecular level on the hepatocyte surface membrane were helpful for liver injury model with control study.
