Murine enteroids culture system and Marmota Himalayana Hepatovirus replication by the enteroids
10.3760/cma.j.issn.1003-9279.2017.06.017
- VernacularTitle: 小鼠肠道类组织三维培养体系的建立及用于喜马拉雅旱獭甲型肝炎病毒增殖研究
- Author:
Lin LIN
1
;
Aijun CHEN
2
;
Lili LI
2
;
Jinsong LI
2
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China; Department of HIV/AIDS Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Academy of Preventive Medicine of Shandong University, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014; China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
Murine enteroids;
Three dimensional culture system;
Marmota Himalayana Hepatovirus;
Hepatitis A virus;
Virus replication
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(6):558-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish mouse intestinal tissue and its application in the proliferation of Marmota Himalayana hepatovirus (MHHAV).
Methods:The intestinal crypts of Balb/C mice were digested with EDTA at a high concentration, cultured in Matrigel and passaged into frozen intestinal enteroids. The monolayers were inoculated on 96-well plates and infected with MHHAV. Real-time qPCR virus concentrations at different time points were compared with those of a number of cell lines routinely used to develop hepatitis A virus.
Results:The mouse intestinal tissue culture system was successfully established. MHHAV did not proliferate in the cell lines commonly used for the culture of hepatitis A virus, but proliferated well in the intestinal tissues of mice. The virus multiplied about 3 700 fold in 4 days.
Conclusions:Mice intestinal enteroids can be used for the isolation and culture of MHHAV.
