VP1 characteristics of coxsakievirus B4 isolated in Henan province
10.3760/cma.j.issn.1003-9279.2017.06.005
- VernacularTitle: 柯萨奇病毒B组4型河南分离株VP1区基因特征分析
- Author:
Yuling XU
1
;
Haixia WANG
;
Haiyan WEI
;
Xueyong HUANG
Author Information
1. Department of Infectious Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention; Henan Key Laboratory of Pathogenic Organism, Zhengzhou 450016, China
- Publication Type:Journal Article
- Keywords:
Coxsakievirus B4;
Hand, foot and mouth disease;
VP1 gene;
Phylogenic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(6):504-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the VP1 region characteristics of coxsakievirus B4 (CoxB4) strain isolated from patients with hand, foot and mouth disease (HFMD) in Henan province and to compare the similarity and difference among these viruses isolated from different diseases.
Methods:The 134 samples were tested by reverse-transcription polymerase chain reaction (RT-PCR) and cell-culture-based isolation of CoxB4. The VP1 of 8 CoxB4 isolated was amplified using specific primers. The sequences were spliced, edited, analyzed and mapped into phylogenetic tree by bioinformatics software ATGC, lustalx1.83, BioEdit and MEGA4.0, respectively.
Results:The VP1 region length of Henan CoxB4 isolates were 852 bp. Compared with other CoxB4 strains released in GeneBank, the similarities of nucleotide and amino acid is 80.3%-97.0%, 96.1%-100%. The largest difference was 19.7% between Henan isolates and diabetes isolates, and 3.0% was the smallest difference with HFMD isolates. Phylogenetic tree analysis showed that Henan CoxB4 isolates belonged to Genotype V, and the isolates from HFMD were close to those of meningitis, distributed in a cluster. These isolates from diabetic patients were in another cluster.
Conclusions:There were Genotype V spreading and circulating of CoxB4 that infected HFMD patients in Henan.
