A preliminary study of three-dimensional bio-printing by polycaprolactone and periodontal ligament stem cells
10.3760/cma.j.issn.1002-0098.2017.04.009
- VernacularTitle: 人牙周膜干细胞与聚己内酯支架的三维生物打印初探
- Author:
Juan XU
1
;
Min HU
1
Author Information
1. Department of Oral and Maxillofacial Surgery, General Hospital of Chinese PLA, Beijing 100853, China
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Tissue scaffolds;
Three-dimensional printing;
Polycaprolactone
- From:
Chinese Journal of Stomatology
2017;52(4):238-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the technical scheme of three-dimensional (3D) bio-printing by polycaprolactone (PCL) and periodontal ligament stem cells (PDLSC).
Methods:To manufacture a 3D bio-printing body, PDLSC were used as seed cells, and polycaprolactone (PCL) was used as the 3D printing scaffold material. Print size was designed at 13.0 mm×13.0 mm, and mesh size was 0.25 mm×0.25 mm (group A) and 0.75 mm×0.75 mm (group B). Cell counting kit-8 was used to detect the proliferation of PDLSC on day 1, day 3 and day 5 respectively. The state of the cells in the 3D printing structure was observed by scanning electron microscope (SEM). Osteoblastic ability of the 3D printing mixture was observed after 14 days of culture by alizarin red mineralized nodule staining method.
Results:Using PDLSC as seed cells and PCL as a scaffold to print two mesh-sized 3D bodies. The body thickness and porosity of group A and group B were 1.1 mm, 1.5 mm and 49.3%, 72.5% respectively. SEM showed that PDLSC proliferated significantly on two sets of 3D structure which was more obvious in group A. In vitro osteogenic induction, a large number of red mineralized nodules formed on the 3D structure.
Conclusions:A 3D structure with a self-defined shape and size was successfully printed using 3D bio-printing equipment. PDLSC can grow and proliferate on the structure.
