Analysis of human papillomavirus infection and p53 expression in patients with hypopharynx squamous cell carcinoma
10.3760/cma.j.issn.1003-9279.2018.06.016
- VernacularTitle: 下咽部鳞状细胞癌患者人乳头瘤病毒感染及p53蛋白表达的检测分析
- Author:
Wei WEI
1
;
Yuntao SONG
1
;
Guohui XU
1
;
Kang XIAO
2
;
Qi SHI
2
Author Information
1. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Head and Neck Surgery Department, Peking University Cancer Hospital & Institute, Beijing 100142, China
2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Hypopharynx squamous cell carcinoma;
Human papillomavirus;
p53
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(6):632-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the infection of human papillomavirus and expression of p53 in patients with hypopharynx squamous cell carcinoma.
Methods:The pathological samples of 15 clinically diagnosed hypopharynx cancer patients were collected. Lunimex and PCR techniques were used to detect the human papillomavirus (HPV) gene and immunohistochemical method was used to analyze the HPV protein expression in the samples. PCR and direct sequencing were used to screen the mutations in p53 gene and immunohistochemistry was used to evaluate the expressing profiles of p53 proteins.
Results:In the 15 cases, 6 were positive for HPV16/18 gene and 2 cases were positive for HPV16/18 E6 protein expression. The total positive rate was about 46.7% (7/15). In addition, 9 of 15 (60.0%) cases had sequencing-confirmed p53 mutations and p53 immunohistochemistry (IHC) confirmed 9 (60.0%) cases had positive staining.
Conclusions:The high HPV infection rate and p53 expression in hypopharynx squamous cell carcinoma patients in the study lays a foundation for further elucidating the pathogenesis of this kind of cancer.
