Advances in the nucleic acid detection method integrated with propidium monoazide to determine the viral infectivity
10.3760/cma.j.issn.1003-9279.2018.05.023
- VernacularTitle: 测定病毒感染性的PMA-核酸检测法的研究进展
- Author:
Yan ZHAO
1
Author Information
1. Animal Biosafety Level 3 Laboratory/the Office of Laboratory Biosafety Commission, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
- Publication Type:Review
- Keywords:
Propidium monoazide;
Nucleic acid;
Viral infectivity
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(5):557-560
- CountryChina
- Language:Chinese
-
Abstract:
Currently, a number of analytical method, such as animal infection test, cell culture assay and polymerase chain reaction, are available for virus detection in clinical samples. The nucleic acid detection method has the highest sensitivity and specificity, but it was not established that the correlation between the detected viral genome and viral infectivity. The nucleic acid detection method integrated with propidium monoazide (PMA) can remedy this shortcoming to determine the viral infectivity. This review firstly describes the principle and procedure of the nucleic acid detection method integrated with PMA. It secondly analyzes the advantages and limitations of the nucleic acid detection method integrated with PMA. Finally, it discusses the influence of the viral structure, the inactivation mode and experimental conditions on the nucleic acid detection method integrated with PMA and summarise the progress of the method.
