Analysis of the VP1 genetic sequence characteristics of EV-A71 and CV-A16 in Dongcheng District, Beijing, 2017
10.3760/cma.j.issn.1003-9279.2018.05.012
- VernacularTitle: 2017年北京市东城区EV-A71和CV-A16分离株VP1基因序列特征分析
- Author:
Lianjun WANG
1
;
Yanyu LI
Author Information
1. Beijing Dongcheng District Center for Diseases Control and Prevention, Beijing, 100050, China
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
Coxsackievrius A16;
VP1;
Phylogemetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(5):505-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the VP1 genetic sequences of Enterovirus A group 71 (EV-A71) strains and Coxsackievirus A group 16 (CV-A16) strains from suspected hand, foot, and mouth disease(HFMD) cases in Dongcheng District, Beijing in 2017, in order to provide basic data for the prevention and control of HFMD.
Methods:By RT-PCR, the VP1 sequences of EV-A71 and CV-A16 VP1 were amplified, and those sequences were compared and analyzed with DNAstar 5.0, while the phylogenetic tree was constructed with MEGA 6.0 software.
Results:Totally 95 throat swabs of suspected HFMD cases were detected in Dongcheng District, 2017. Forty-six (48.42%) specimens were positive for enterovirus, 4 (4.21%) specimens were positive for EV-A71 and 4 (4.21%) specimens were positive for CV-A16, 38 (40.00%) specimens were positive for non-EV-A71, non-CV-A16 enterovirus. The nucleotide and amino acid sequence homology of the four EV-A71 strains were respectively 92.89%-99.15% and 97.32%-98.66%. The nucleotide and amino acid sequence homology of the three CV-A16 strains were respectively 93.90%-98.76% and 98.32%-100.00%. The phylogenetic tree indicated that EV-A71 strains belonged to C4a subtype and CV-A16 strains belonged to B1b subtype.
Conclusions:The main pathogens causing HFMD were non-EV-A71 and non-CV-A16 enteroviruses in Dongcheng District of Beijing in 2017, but EV-A71 and CV-A16 still account for a certain proportion. The EV-A71 strains belong to C4a genetic subtype; the CV-A16 strains belong to B1b genetic subtype. The subtypes of gene matched with the dominant strains in most areas of China in recent years and no new subtypes were detected.
