NEDDylation is involved in hepatitis B virus pregenomic RNA encapsidation
10.3760/cma.j.issn.1003-9279.2018.05.002
- VernacularTitle: NEDDylation参与乙型肝炎病毒前基因组RNA的包装
- Author:
Cheng LI
1
;
Jing ZHANG
;
Yongxiang WANG
Author Information
1. Key Laboratory of Medical Molecular Virology, Ministry of Education and Ministry of Public Health, Shanghai Medical College of Fudan University, Shanghai 200032, China
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
NEDDylation;
Replication;
pgRNA encapsidation
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(5):458-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of an ubiquitin-like modification NEDDylation on HBV life cycle.
Methods:Cells were incubated with various concentrations of MLN4924, which is a selective inhibitor of neural precursor cell-expressed developmentally down regulated 8 (NEDD8) activating enzyme (NAE), to block cellular protein NEDDylation. And a series concentration of lamivudine (LAM), an inhibitor of HBV polymerase was set as control. Then we tested HBV DNA replication, protein expression and nucleocapsids formation. Huh-7 cells were transfected with polymerase-inactivated HBV expression plasmid, then incubated with 0.1 μmol/L MLN4924 or çvehicle-DMSO. Then we tested capsids and capsid-associated RNA.
Results:MLN4924 inhibited HBV replication in a dose-dependent manner (0~2 μmol/L). When the concentration of MLN4924 was 0.1 μmol/L, the amount of nucleocapsids in the cells did not change significantly, however the encapsidation of HBV pregenomic RNA (pgRNA) decreased by about 47%.
Conclusions:NEDDylation is involved in hepatitis B virus pregenomic RNA encapsidation.
