Application of nanopore sequencing technology in viral genome research
10.3760/cma.j.issn.1003-9279.2018.02.024
- VernacularTitle: 纳米孔测序技术在病毒基因组研究中的应用
- Author:
Ji WANG
1
;
Xuejun MA
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Review
- Keywords:
Nanopore sequencing;
MinION;
Viral genome sequencing;
Viral detection
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):214-219
- CountryChina
- Language:Chinese
-
Abstract:
MinION is a commercial nanopore sequencer developed by Oxford Nanopore Techno-logies. Compared with the second-generation sequencing platform, MinION is characterized by ultra-long reads, simple and fast sequencing, high portability and real-time data analysis. MinION has been widely applied to the fields of virus identification, whole genome sequencing, new virus discovery, quasispecies and virus evolution. In this article, we review the application of nanopore sequencing technology in the research of the viral genome in recent years, and discuss the existing problems. We also present the prospect for the development trend of nanopore sequencing in the future.
