Detection and clinical significance of differentially expressed microRNAs in chronic hepatitis B patients before being treated with pegylated interferon
10.3760/cma.j.issn.1003-9279.2018.02.010
- VernacularTitle: CHB患者PegIFN治疗前差异表达microRNAs的检测及意义
- Author:
Yanlin YANG
1
;
Ming LIU
;
Ying DENG
;
Yan GUO
;
Xuqing ZHANG
;
Dedong XIANG
;
Li JIANG
;
Zhonglan YOU
;
Yi WU
;
Maoshi LI
;
Qing MAO
Author Information
1. Institute of Infectious Disease, Southwest Hospital, Army Medical University, Third Military Medical University; the Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing 400038, China
- Publication Type:Journal Article
- Keywords:
Pegylated interferon;
Peripheral blood mononuclear cells;
MicroRNAs;
HBsAg
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):155-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect differentially expressed microRNAs in chronic hepatitis B (CHB) before being treated with pegylated interferon (PegIFN) and the relationship between their target genes and HBsAg loss.
Methods:Pretreatment differentially expressed microRNAs between different response groups were screened using high throughput microarrays and validated by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Bioinformatics analysis was performed to determine their target genes potential mechanistic roles.
Results:A total of 417 microRNA were differentially expressed between different response groups, among which 342 were up-regulated and 75 were down-regulated. miR-3960, miR-126-3p, miR-23 a-3p and miR-335-5p were verified to be down-regulated by RT-qPCR result in HBsAg loss group. Bioinformatic analysis result show that the relevant pathways of microRNAs include AMPK signal pathway, NOD-like signal pathway, NF-kappa B signal pathway and mTOR signal pathway.
Conclusions:HBsAg loss is probably achieved as the result of genes expression regulated in association with immune response, further enhance the immune response of HBV elimination and acquire HBsAg loss.
