Study on the regulation of microRNA-375 on PDK1 expression in brain tissue of scrapie agent 139 A infected mice
10.3760/cma.j.issn.1003-9279.2018.01.006
- VernacularTitle: 羊瘙痒因子感染小鼠脑组织中MicroRNA-375对PDK1蛋白表达调控研究
- Author:
Qiang SHI
1
;
Lina ZHANG
;
Jing WANG
;
Chen GAO
Author Information
1. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Scrapie agen139A;
microRNA-375;
3’-phosphoinositide-dependent kinase 1;
Prion disease
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation of microRNA-375(miR-375) on the expressions of 3’-phosphoinositide-dependent kinase 1 (PDK1) in the brain tissues of scrapie agent 139 A infected mice.
Methods:PDK1 protein in 139 A infected mice brain tissue was detected by WB and immunochemistry. The change of microRNA-375 was detected by reverse transcription polymerase chain reaction (RT-PCR) and next-generation sequencing method . The pmiR-REPORT reporter system was used to value the regulation of miR-375 on PDK1 3’-untranslated region (3’UTR).
Results:The expression of PDK1 in the brain tissue of 139 A infected mice was significantly increased as compared to that of control group, while the expression of miR-375 was decreased. The result of pmiR-REPORT reporter system showed that PDK1 3’UTR was the regulation target of miR-375.
Conclusions:The expression of PDK1 in the brain tissue of 139 A infected mice was significantly increased, which was probably related to the regulation of miR-375 on the 3’UTR of PDK1.
