Current status of cytomegalovirus infection in pregnant women and its correlation with pregnancy outcomes in Huzhou
10.3760/cma.j.issn.1003-9279.2019.06.012
- VernacularTitle: 湖州市妊娠期妇女巨细胞病毒感染现状及与妊娠结局相关分析
- Author:
Juan YAO
1
;
Rong FANG
2
;
Guoping SHEN
1
;
Guosong SHEN
1
;
Su ZHANG
2
;
Yan LU
2
;
Haiyan LI
1
Author Information
1. Department of Clinical Laboratory, the Maternity and Child Health Cave Hospital of Huzhou, Huzhou 313000, China
2. Department of Obstetrics and Gynecology, the Maternity and Child Health Care Hospital of Huzhou, Huzhou 313000, China
- Publication Type:Journal Article
- Keywords:
Cytomegalovirus infection;
Polymerase chain reaction;
Enzyme-linked immunosorbent assay;
Antibody affinity;
Pregnancy outcome
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):617-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the current situation of human cytomegalovirus(HCMV) infection in local pregnant women and its impact on pregnancy outcomes, so as to provide reference for strengthening health care during pregnancy and promoting the policy of "fewer and better children"
Methods:A total of 2 000 pregnant women underwent prenatal examination who had delivery in the Maternal and Child Health Hospital of Huzhou from July 2017 to December 2018. HCMV IgM, IgG and IgG- avidity index (AI) were detected by enzyme-linked immunosorbent assay (ELISA), and HCMV DNA was detected by real-time polymerase chain reaction (PCR) -fluorescent probe. We also analyzed the positive status of HCMV and its relationship with pregnancy outcome.
Results:Among the 2 000 pregnant women, 1 941 cases (97.05%) were positive for HCMV IgG, 26 cases (1.30%) had active infection with HCMV, of whom 5 cases (0.25%) were serum HCMV IgM/IgG dual positive, 24 cases (1.20%) were urine HCMV DNA positive. HCMV IgG AI test result showed that 3 cases of AI ≤30%, which means that they had primary infections. The detection rates of HCMV DNA positive and HCMV IgM/IgG dual positive were 12.12%, 4.54% in 66 pregnant women with adverse pregnancy and 0.83%, 0.10% in 1 934 pregnant women with normal pregnancy. The difference was significant (χ2=68.663, 50.499, P<0.01). Logistic regression analysis showed that HCMV IgM/IgG dual positive (OR=12.743, 95%CI: 1.202-135.100, P=0.035), HCMV-DNA positive (OR=10.426, 95%CI: 3.635-29.909, P<0.01) were independent risk factors for adverse pregnancy outcomes. In addition, low education level, living in rural areas and having a history of adverse pregnancy also increased the incidence of adverse pregnancy outcomes.
Conclusions:HCMV infection is prevalent in pregnant women in this area, but only a few of them had active infection. HCMV active infection is a risk factor for adverse pregnancy outcomes.
