Exploratory research on detection of influenza viruses by reverse transcriptase-recombinase polymerase amplification
10.3760/cma.j.issn.1003-9279.2019.05.019
- VernacularTitle: 重组酶聚合酶扩增技术在流感病毒检测中的探索性研究
- Author:
Ning SUN
1
;
Juan YU
2
;
Xiaoling YAN
1
;
Deyu GAO
1
;
Bo CHEN
3
;
Weiping WANG
1
;
Xiaojun LI
1
Author Information
1. Department of Clinical Laboratory Science, Nanjing General Hospital of People's Liberation Army, Nanjing 210002, China
2. Department of Clinical Laboratory Science, Nanjing General Hospital of People's Liberation Army, Nanjing 210002, China; Nanjing Lishui People′s Hospital, Zhongda Hospital Lishui Branch, Southeast University, Nanjing 210000, China
3. Ningbo Health Gene Technologies Co. Ltd., Ningbo 315040, China
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Detection;
Subtyping;
Recombinase polymerase amplification
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):530-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish and hevaluate a detection method for influenza virus using reverse transcriptase-recombinase polymerase amplification (RT-RPA).
Methods:RT-RPA was developed for detection of influenza viruses (type A and B) and subtyping of H1 and H3 using the primers targeted matrix and hemagglutinin (HA) genes of influenza A virus, and non-structural (NS) protein gene of influenza B virus. The specificity and sensitivity of RT-RPA were determined.
Results:The RT-RPA for the detection of influenza viruses showed specific amplification products of corresponding target gene, but no amplification products for other respiratory viruses, indicating that the method had good specificity. The detection limits of RT-RPA were 100 copies/μl. RT-RPA combined with SYBR Green I was used for the detection of influenza B virus with the detection limit of 100 copies/μl.
Conclusions:The feasibility of detecting influenza virus by RT-RPA was preliminarily confirmed.
