EV71 induces incomplete cellular autophagy to promote self-replication
10.3760/cma.j.issn.1003-9279.2019.05.001
- VernacularTitle: EV71诱导不完整的细胞自噬促进自身复制
- Author:
Ziwei LIU
1
;
Wenqiang ZHANG
2
;
Shaoxia SONG
2
;
Li ZHAO
1
;
Zhiyu WANG
1
;
Hongling WEN
1
Author Information
1. Department of Microbiological Laboratory Technology, School of Public Health, Shandong University, Key Laboratory for the Prevention and Control of Infectious Diseases (Key Laboratory of the " 13th Five-Year Plan" University of Shandong), Jinan 250012, China
2. Shandong Center for Disease Control and Prevention; Shandong Provincial Key Laboratory of Infectious Disease Prevention and Control, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
Autophagy;
Viral replication;
mTOR
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):449-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between enterovirus 71 (EV71) and autophagy and the effect of autophagy on viral replication.
Methods:RD cells were pretreated with rapamycin, Chloroquine (CQ) and 3-methyladenine (3-MA). The effects of different stages of autophagy on viral replication were detected by Western blotting and plaque assay.
Results:The study showed that EV71 infection could induce incomplete autophagy in RD cells. Replication of EV71 was promoted in the CQ treatment group, while the level of replication of the rapamycin and 3-MA treatment groups was reduced. Further studies have shown that mTOR is a key molecule affecting EV71 replication, and inhibition of mTOR by rapamycin can inhibit the synthesis of viral RNA.
Conclusions:The effects of different stages of autophagy on viral replication are also different. Inhibition of autophagic lysosome degradation promotes viral replication, while inhibition of the early stage of autophagy or promotion of autophagy reduces the level of viral replication. mTOR can affect the replication of EV71 at RNA level.
