Effects of cyclic mechanical stretch on BMP-2 expression in RA and OA fibroblast-like synoviocytes
- VernacularTitle:周期性机械拉伸对类风湿关节炎和骨关节炎成纤维样滑膜细胞BMP-2表达的影响
- Author:
Wei ZHOU
1
;
Yong-Gang LÜ
2
;
K GURINDER
3
;
Yan YAN
4
;
Wan-qian LIU
5
;
Ning-ning YANG
6
;
Fei-fei ZHANG
7
;
Li YANG
8
Author Information
1. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
2. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “112” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
3. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “113” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
4. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “114” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
5. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “115” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
6. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “116” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
7. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “117” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
8. Key Laboratory of Biorheological Science and Technology, Ministry of Education; “118” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University
- Publication Type:Journal Article
- Keywords:
Cyclic stretch;
Mechanical stimulation;
Tensile stress;
Rheumatoid arthritis(RA);
Osteoarthritis(OA);
Fibroblast like synoviocytes (FLS);
Bone morphogenetic protein 2 (BMP-2);
Inflammatory cytokines;
Pathology
- From:
Journal of Medical Biomechanics
2011;26(2):E121-E127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of mechanical stimulation in synovium under different pathological conditions through studying the effects of cyclic mechanical stretch on the expression of bone morphogenetic protein 2 (BMP-2) in rheumatoid arthritis (RA) and osteoarthritis (OA) fibroblast-like synoviocytes (FLS). Method 6% and 0.5 Hz stretch generated by Flex cell 4000 tension systems was applied on normal, RA and OA FLS of human knee joint source under normal and inflammatory conditions for 2 h or 6 h, respectively. Results Cyclic mechanical stretch of 6%, 0.5 Hz had no significant effects on the expression of BMP-2 in normal, RA and OA FLS at 2 h, while in RA FLS it increased significantly at 6 h. Inflammatory cytokines (IL-1β) didn’t influence normal FLS at 2 h, but made BMP-2 mRNA significantly increased at 6 h. IL-1β increased BMP-2 mRNA of RA FLS significantly both at 2 h and 6 h. IL-1β increased BMP-2 mRNA of OA FLS significantly only at 2 h, but had no significant effect at 6 h. The co-effect of IL-1β and cyclic mechanical stretch induced the ascension of BMP-2 expression significantly in normal and RA FLS at 2 h, and in normal, RA and OA FLS at 6 h. Conclusions Response of BMP-2 mRNA to mechanic stimulation and IL-1β in normal, RA and OA FLS were different. Inflammation may play a more important role than mechanical stimulation in the pathogenesis of RA and OA. Synergetic effect in inflammation and mechanical stimulation were found in OA FLS at 6 h, which reveals that they may co-act in the occurrence and development of OA.
