Effects of centrifugating pressure on the function of preosteoblast OCT-1
- VernacularTitle:离心加压对前成骨细胞株OCT-1成骨功能的影响
- Author:
Qin BIAN
1
;
Zhu YANG
2
;
Shu feng LIU
2
;
Xue jun CUI
2
;
Qian qian LIANG
2
;
Yong jian ZHAO
2
;
Sheng LU
2
;
Qi SHI
2
;
Yong jun WANG
2
Author Information
1. Longhua Hospital, Shanghai University of Traditional Chinese Medcine;Institute of Spine, Shanghai Traditional Chinese Medcine Institute;Institute of Integrated Medcine, Huashan Hospital, Fudan University
2. Longhua Hospital, Shanghai University of Traditional Chinese Medcine;Institute of Spine, Shanghai Traditional Chinese Medcine Institute
- Publication Type:Journal Article
- Keywords:
Gravity;
Centrifugation;
Cell pressure;
Cell culture
- From:
Journal of Medical Biomechanics
2010;25(6):E449-E455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of simulating gravity pressure and observe its effect on preosteoblast OCT 1 by centrifugation. Method OCT-1 cells were cultured in 1% agarose gel with a final concentration of 2×107 cells per millilitre. The experiment was divided into two groups according to the duration: one day (1 d) and five days (5 d). Each group was further divided into three subgroups: 0 r/min (control), 200 r/min, and 500 r/min. The 200 r/min group and 500 r/min group were centrifuged for three hours once a day for one day or five days respectively. The control group was kept in the same environment without centrifugation. Results The ColⅠpositive staining was slightly strengthened in the 1d 200 r/min group while it was even more strengthened in the 1d 500 r/min group. Conversely, the positive staining was stronger in the 5d 200 r/min group than that in the 5d 500 r/min group. The markers related to osteoblast differentiation such as Alkaline phosphatise (ALP), Type I collagen α2 (Col1α2), Osteocalcin (OC) and runt-related transcription factor-2 (Runx2) mRNA expression were all up regulated after centrifugation. ConclusionsCentrifugation is a practical method for cell pressure and plays a significant role in promoting the differentiation of preosteoblast OCT-1, which can be used as a new method to simulate the gravity.
