Diagnosis of intellectual disability/global developmental delay via genetic analysis in a central region of China
10.1097/CM9.0000000000000295
- Author:
Li-Hong LIAO
1
;
Chen CHEN
2
;
Jing PENG
2
;
Li-Wen WU
2
;
Fang HE
2
;
Li-Fen YANG
2
;
Ci-Liu ZHANG
2
;
Guo-Li WANG
2
;
Pan PENG
2
;
Yu-Ping MA
2
;
Pu MIAO
2
;
Fei YIN
2
Author Information
1. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;Hunan Intellectual and Developmental Disabilities Research Center, Changsha, Hunan 410008, China;Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430071, China
2. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;Hunan Intellectual and Developmental Disabilities Research Center, Changsha, Hunan 410008, China
- Publication Type:Journal Article
- Keywords:
Intellectual disability;
Global developmental delay;
Children;
Gene analysis;
Etiology
- From:
Chinese Medical Journal
2019;132(13):1533-1540
- CountryChina
- Language:English
-
Abstract:
Background:Advanced technology has become a valuable tool in etiological studies of intellectual disability/global developmental delay (ID/GDD). The present study investigated the role of genetic analysis to confirm the etiology in ID/GDD patients where the cause of the disease was uncertain in central China.
Methods:We evaluated 1051 ID/GDD children aged 6 months to 18 years from March 2009 to April 2017. Data concerning basic clinical manifestations were collected, and the method of etiology confirmation was recorded. Genome-wide copy number variations (CNVs) detection and high-throughput sequencing of exons in the targeted regions was performed to identify genetically-based etiologies. We compared the incidence of different methods used to confirm ID/GDD etiology among groups with differing degrees of ID/GDD using the Chi-square or Fisher exact probability test.
Results:We recruited 1051 children with mild (367, 34.9%), moderate (301, 28.6%), severe (310, 29.5%), and profoundly severe (73, 6.9%) ID/GDD. The main causes of ID/GDD in the children assessed were perinatal factors, such as acquired brain injury, as well as single gene imbalance and chromosomal gene mutation. We identified karyotype and/or CNVs variation in 46/96 (47.9%) of cases in severe ID/GDD patients, which was significantly higher than those with mild and moderate ID/GDD of 34/96 (35.4%) and 15/96 (15.6%), respectively. A total of 331/536 (61.8%) patients with clear etiology have undergone genetic analysis while 262/515 (50.9%) patients with unclear etiology have undergone genetic analysis (χ2 = 12.645, P < 0.001). Gene structure variation via karyotype analysis and CNV detection increased the proportion of children with confirmed etiology from 51.0% to 56.3%, and second-generation high-throughput sequencing dramatically increased this to 78.9%. Ten novel mutations were detected, recessive mutations in X-linked genes (ATPase copper transporting alpha and bromodomain and WD repeat domain containing 3) and dominant de novo heterozygous mutations in X-linked genes (cyclin-dependent kinase like 5, protocadherin 19, IQ motif and Sec7 domain 2, and methyl-CpG binding protein 2) were reported in the study.
Conclusions:The present study indicates that genetic analysis is an effective method to increase the proportion of confirmed etiology in ID/GDD children and is highly recommended, especially in ID/GDD children with uncertain etiology.
