Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis
10.13422/j.cnki.syfjx.20182492
- VernacularTitle: 基于灰色关联分析探索艾纳香非挥发性部位的抗炎活性谱效关系
- Author:
Yang WANG
1
;
Xiao PENG
1
;
Yan-ling HE
1
;
Xu SUN
1
;
Xue MA
1
;
Guang-cheng WANG
1
;
Yan-yu LAN
1
;
Yong-jun LI
1
Author Information
1. School of Pharmaceutical Sciences, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
Blumea balsamifera;
caffeoylquinic acid derivates;
anti-inflammation;
gray correlation analysis;
spectrum-effect relationship;
fingerprint;
n-butanol site
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):180-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the correlation between UPLC fingerprint of anti-inflammatory effect of active components from nonvolatile fraction of Blumea balsamifera, and to provide the basis for clarifying the anti-inflammatory material basis of B. balsamifera. Method: UPLC was used to establish fingerprint of nonvolatile fraction of 12 batches of B. balsamifera and their common fingerprint peaks were identified by UPLC-Q-TOF-MS.The corresponding pharmacodynamic data were obtained by auricle swelling and inflammation model mice induced by xylene, and spectrum-effect relationship was established by gray correlation analysis. Result: A total of 14 common peaks in nonvolatile fraction of B. balsamifera were established by UPLC fingerprint and 9 common peaks of them were identified.The correlation between UPLC fingerprint and the anti-inflammatory activity was from 0.717 1 to 0.550 5.The contribution of chemical compositions represented by each characteristic peak to the anti-inflammatory efficacy was in the order of peak 3 > peak 9 > peak 4 > peak 11 > peak 2 > peak 1 > peak 14 > peak 7 > peak 6 > peak 5 > peak 12 > peak 8 > peak 10 > peak 13, and the top two peaks with strong contribution to anti-inflammatory effect were peak 3 and peak 9, they were 3-O-caffeoylquinic acid and 3, 5-di-O-caffeoylquinic acid identified by contrast reference substances, respectively. Conclusion: The active substances in nonvolatile fraction of B. balsamifera are obtained through the study on the relationship between spectrum and efficiency, and the anti-inflammatory efficacy of the nonvolatile fraction is the result of combination of various components.It is clear that the caffeoylquinic acid derivates act as predominant anti-inflammatory active substance of nonvolatile fraction of B. balsamifera.
