Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra
10.13422/j.cnki.syfjx.20182304
- VernacularTitle: 基于HPLC-DAD特征图谱分析莱菔子饮片酶解过程中化学成分的变化
- Author:
Li-qiao ZHU
1
;
Shao-hua YU
1
;
Xi ZHANG
1
;
Hong-lei ZHOU
1
;
Hua-gang SHENG
1
Author Information
1. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China
- Publication Type:Research Article
- Keywords:
Raphani Semen;
enzymolysis time;
characteristic spectrum;
ultraviolet absorption spectrum;
sinadiosides;
glucoraphenin;
sinapine thiocyanate
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):140-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the dynamic changes of components in the enzymolysis process of raw products of Raphani Semen. Method: HPLC was employed to analysis of characteristic spectra of Raphani Semen at different enzymolysis time with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution and detection wavelength at 225 nm.The characteristic peaks were calibrated, meanwhile, the UV spectra of characterstic peaks were extracted, and the difference between UV spectra and the changes of peak areas were compared, and the dynamic changes of characterstic components in Raphani Semen were analyzed. Result: Eleven characteristic peaks were marked from the characteric spectra of raw products of Raphani Semen at different enzymolysis time, and glucoraphenin and sinapine thiocyanate were assigned.Glucoraphenin was enzymatically hydrolyzed fastly by myrosinase, and an intermediate was generated, and then continue to be decomposed into other components.Sinapine thiocyanate did not change significantly during the enzymolysis process, and sinadiosides was also enzymatically degraded. Conclusion: The enzymolysis of Raphani Semen is not only the glucoraphenin, but also the sinadiosides.This paper can provide reference for the property change of Raphani Semen in processing.
