Effect of Huayu Qutan Recipe in Regulating Aortic MAPK Pathway-related Gene Expression in Atherosclerosis Mice
10.13422/j.cnki.syfjx.20190291
- VernacularTitle: 化瘀祛痰方对ApoE-/-小鼠主动脉MAPK信号通路相关蛋白表达的影响
- Author:
Xue LENG
1
;
Yang LI
1
;
Nan SONG
1
;
Ying WANG
1
Author Information
1. Key Laboratory of Ministry of Education for Traditional Chinese Medicine(TCM) Viscera-State Theory and Applications, Liaoning University of TCM, Shenyang 110847, China
- Publication Type:Research Article
- Keywords:
atherosclerosis;
ApoE-/- mice;
aorta;
enzyme-linked immuno sorbent assay(MAPK) signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):116-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression changes of mitogen activated protein kinase(MAPK) signaling pathway-related genes in ApoE-/- mice and the intervention effect of Huayu Qutan recipe on them, in order to explore the anti-atherosclerotic(AS) effect and possible mechanism of Huayu Qutan recipe. Method: Fifty ApoE-/- mice were randomly divided into model group, tanshinone ⅡA group (30 mg·kg-1·d-1), and high-dose phlegm group (20 g·kg-1·d-1), the middle-dose group (10 g·kg-1·d-1), and the low-dose group (5 g·kg-1·d-1), and 10 C57BL/6/J mice were included in the blank controls group. Automated biochemical analyzer was used to detect serum triglyceride(TG), cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) content; hematoxylin-eosin(HE) was used to detect aortic plaque in each group; oil red O was used to detect liver lipid deposition in each group; enzyme-linked immuno sorbent assay(ELISA) was used to determine vascular cell adhesion protein-1(VCAM-1), intercellular adhesion molecule-1(ICAM-1), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) in serum of each group; Western blot was performed to detect c-Jun N-terminal kinase (JNK), phosphorylated c-Jun N-terminal kinase(p-JNK), c-Jun N-terminal kinase(JNK), p38 MAPK, p-p38 MAPK, extracellular regulated protein kinases (ERK), and expression of p-ERK. Result: Compared with the blank control group, serum TC, TG, LDL-C levels in the model group were significantly increased, while HDL-C levels were significantly decreased; aortic plaques were observed in the aortic lumen, and lots of lipid deposition were observed in the liver cells. Serum inflammatory factors TNF-α, IL-6, VCAM-1, ICAM-1 increased(P<0.05); and intravascular associated MAPK signaling pathway p-p38 MAPK, p-JNK gene expressions increased significantly(P<0.05). Compared with the model group, TC, TG, LDL-C in the tanshinone ⅡA group and the phlegm group were significantly decreased, HDL-C was significantly increased, and atherosclerotic plaque area and liver lipid were remarkably reduced in the aortic lumen. The deposition amount was significantly reduced, and the levels of inflammatory factors such as TNF-α, IL-6, VCAM-1 and ICAM-1 were decreased. The expressions of p-p38 MAPK and p-JNK genes in intravascular associated MAPK signaling pathway were significantly decreased. p-ERK expression trend was not obvious(P<0.05). Conclusion: Huayu Recipe can inhibit the formation of aortic plaque in ApoE-/- mice, and its mechanism may be related to the regulation of vascular MAPK signaling pathway gene expression.
