Mechanisms and Components Identification of Naoxintong Intestinal Absorption Liquid Protecting Cardiac Injury Induced by H2O2
10.13422/j.cnki.syfjx.20190407
- VernacularTitle: 脑心通胶囊肠吸收液保护过氧化氢致H9c2心肌细胞损伤的作用机制及有效药味筛选
- Author:
Zi-long HE
1
;
Ye ZHAO
1
;
Yi ZHANG
1
;
De-feng LI
1
;
Fang-bo ZHANG
1
;
Hong-jun YANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Naoxintong capsule;
intestinal absorption liquid;
cardiac injury;
hydrogen peroxide;
active medicinal identification
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):29-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Intestinal absorption liquid as medicine carriers, one or more kinds of traditional Chinese medicine protecting cardiac injury in Naoxintong capsule will be found through separation and combination of prescription. This study also can expand protecting mechanisms of Naoxintong capsule. Method: Naoxintong intestinal absorption liquids of single, combination and total prescription were prepared. H9c2 cell line exposed to H2O2 was established. Cell survival rate was determined with methyl thiazolyl tetrazolium(MTT). Cell apoptosis rate was examined by Annexin Ⅴ-FITC/PI staining with a flow cytometer. Aquaporin1(AQP1) expression was detected by Western blot. One or more kinds of traditional Chinese medicine in Naoxintong capsule which exerted protective effect from cardiac injury were screened through separation and combination of prescription. Result: As compared with control group, the protein expression level of AQP1 was significantly increased(P<0.01), and the apoptotic H9c2 cells increased(P<0.05). Compared with model group, Naoxintong intestinal absorption liquid low, medium and high dose group decreased AQP1 expression(P<0.05), and naoxintong intestinal absorption liquid high dose group inhibited cardiac cells apoptosis(P<0.05), thus protected H9c2 cells from oxidative damages. Salviae Miltiorrhizae Radix et Rhizoma, Pheretima, Scorpio are main constituents which had protective actions in total prescription. Protective effect of those three combination prescription in high dose group was the best(P<0.05), and effect of combination prescription without those three was the second(P<0.05). Conclusion: Naoxintong capsule acts as a protective role in myocardial injury through decreasing AQP1 expression and inhibiting cell apoptosis. Salviae Miltiorrhizae Radix et Rhizoma, Pheretima, Scorpio are important components in Naoxintong capsule.
