Protective Effect of Yinhuangshen Essential Oil on Skin Damage in Mice with Solar Dermatitis
10.13422/j.cnki.syfjx.20190437
- VernacularTitle: 银黄参精油对日光性皮炎模型小鼠皮肤损伤的保护作用
- Author:
Meng-yan LI
1
;
Shuo TIAN
1
;
Ming-san MIAO
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
Yinhuangshen essential oil;
altraviolet(UVB);
external use of traditional Chinese medicine;
solar dermatitis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):13-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Yinhuangshen essential oil in mice of solar dermatitis model by ultraviolet (UVB). Method: Sixty SPF BALB/c female mice were randomly divided into blank group, model group, Minlixiao group, and large and small-dose Yinhuangshen essential oil groups. Except for blank group, model group was given 0.25 mL·cm-2 normal saline on the back every day. The Minlixiao group was given Min Lixiao every day. Low-dose Yinhuangshen essential oil group was given 0.125 mL·cm-2, and large-dose group was given 0.25 mL·cm-2, once a day, and the corresponding drugs were given continuously for 8 days. Except for blank group, all of the other groups were irradiated for 1.5 hours at 15 cm away from 308 nm UVB lamp on the 6th day. One hour and 24 hours after irradiation, mice were given corresponding drug once, and skin was observed 48 hours later. Contents of nitric oxide (NO), nitric oxide synthase (NOS) and tumor necrosis factor-alpha (TNF-α) in some local skin homogenates were measured. The histopathological changes were observed by htoxylin eosin(HE) staining. Result: Compared with blank group, skin of model group was reddish, swollen and ulcerated, epithelium and adnexal were absent, a large number of inflammatory cells and necrosis were adhered to the surface, the local histopathological changes significantly (P<0.01), and NO, NOS, TNF-α were significantly increased (P<0.01). Compared with model group, contents of NO, NOS and TNF-α in the skin tissue of Minlixiao group and Yinhuangshen essential oil group were significantly decreased, and it could remarkable reduce the pathological changes in local tissue morphology (P<0.01). Conclusion: Yinhuangshen essential oil has a good effect in preventing and treating solar dermatitis.
