Effect of Fengshi Qutong Capsule on Human Umbilical Vein Endothelial Cells Induced by VEGF
10.13422/j.cnki.syfjx.20190537
- VernacularTitle: 风湿祛痛胶囊对VEGF诱导的人脐静脉内皮细胞功能的影响
- Author:
Yi-qun LI
1
;
Lian-hua HE
2
;
Chun-fang LIU
2
;
Jing-xia WANG
2
;
Cong-cong SUN
2
;
Yu JING
3
;
Yan-dong MIAO
3
;
Na LIN
1
Author Information
1. Chengde Medical University, Chengde 067000, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Tonghua Golden-horse Group, Beijing 100028, China
- Publication Type:Research Article
- Keywords:
Fengshi Qutong capsule;
human umbilical vein endothelial cells (HUVEC);
proliferation;
migration;
invasion;
adhesion
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):119-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Fengshi Qutong capsule on the migration, adhesion, invasion and tube formation of human synovial cells and the phosphorylation and protein expression of vascular endothelial growth factor receptor 2 (VEGFR2). Method: With human umbilical vein endothelial cells (HUVEC) as the research object, low, middle and high-dose Fengshi Qutong capsule(0.02,0.1,0.5 μg·L-1) on HUVEC was determined by methye thiazolye telrazlium (MTT) colorimetric assay for the follow-up experiment. The transwell migration, adhesion and transwell invasion test were used to detect the migration and adhesion of the different concentrations of Fengshi Qutong capsule in HUVEC. The expression of VEGFR2 in HUVEC was detected by Western blot, and Real-time PCR was used to detect the content of VEGFR2 mRNA in cells. Result: Compared with normal group, the proliferation of HUVEC was significantly increased after 24 h and 48 h of VEGF induction (P<0.01), the migration, adhesion, invasion and lumen formation of HUVEC were significantly increased within 24 h (P<0.01), and VEGFR2 phosphorylation protein and mRNA expression levels in HUVEC were significantly elevated in HUVEC (P<0.01). Compared with VEGF group, 0.02, 0.1 and 0.5 μg·L-1 Fengshi Qutong capsule were administered in vitro for 48 h to inhibit HUVEC proliferation activity in a dose-dependent manner (P<0.05, P<0.01). Within 24 h, VEGF-induced HUVEC migration, adhesion, invasion and lumen formation were significantly inhibited (P<0.05), and negatively regulated VEGFR2 phosphorylation protein and gene expression levels (P<0.05). Conclusion: Fengshi Qutong capsule can inhibit the migration, adhesion, invasion and tube formation of HUVEC. This effect may be related to the inhibition of phosphorylation, and protein and mRNA expression level of VEGFR2.
