Molecular Mechanism of Tangshenping Capsule in Intervening Apoptosis of Podocytes Induced by High Glucose+LPS Through PI3K/Akt Signaling Pathway
10.13422/j.cnki.syfjx.20190421
- VernacularTitle: 糖肾平胶囊通过PI3K/Akt信号通路干预高糖+LPS诱导足细胞凋亡的机制
- Author:
Ying-chao WANG
1
;
Jing ZHAO
2
;
Zong-jiang ZHAO
2
;
Lei DU
2
;
Chen TIAN
2
;
Ling LI
1
Author Information
1. Beijing Aerospace General Hospital, Beijing 100007, China
2. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
diabetic nephropathy;
podocyte;
Tangshenping capsule;
phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):105-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe effect of Tangshenping capsule on lipopolysaccharide (LPS)-stimulated podocytes apoptosis under high glucose conditions of podocyte in rats with diabetic nephropathy (DN), and discuss possible mechanism. Method: With cultured rat podocytes as object of study,the podocytes model was established with high glucose and LPS,and divided into 7 groups:control group,high glucose group,high glucose plus LPS group,irbesartan group,and low,moderate and high-dose Tangshenping capsule groups.Protein and mRNA expressions of CD2-associated protein (CD2AP), nephrin, phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) and B-cell lymphoma-2 associated death protein (Bad protein) of podocyte in each group were detected by Western blot and reverse transcription polymerase chain reaction (RT-PCR). Result: Compared with control group,the protein and mRNA expressions of podocyte CD2AP, nephrin, PI3K, Akt decreased definitely (P<0.05, P<0.01), while Bad protein and mRNA expressions increased definitely (P<0.05, P<0.01). Compared with high glucose plus LPS group,the protein and mRNA expressions of podocyte CD2AP, PI3K, Akt increased definitely (P<0.05,P<0.01), whereas Bad protein and mRNA expressions decreased definitely (P<0.05,P<0.01) in irbesartan group. The protein and mRNA expressions of podocyte nephrin, CD2AP, PI3K, Akt increased definitely (P<0.05,P<0.01), and Bad protein and mRNA expressions decreased definitely (P<0.05, P<0.01) in three Tangshenping capsule groups. Conclusion: Tangshenping capsule can maintain a stable protein expression of Slit diaphragm (SD) in podocyte, inhibit podocyte apoptosis by activating PI3K/Akt signaling pathway. This is one of mechanisms for preventing and treating diabetic nephropathy.
